| Liposcelis tricolor Badonnel is a new recorded species in China, which was found in Heze, Shandong during our investigation of booklice species in grain storage. Liposcelis tricolor belongs to Psocoptera, Family Liposcelididae, and genus Liposcelis. It is an important kind of pest groups infesting stored products and unprocessed flour, and also transmit the bacteria and fungus. Wolbachia is a maternally inherited intracellular bacteria that infect a wide range of arthropods. Wolbachia has attracted much attention because of their ability to manipulate the reproductive biology of their hosts, including cytoplasmic incompatibility (CI), parthenogenesis induction (PI), male-killing and feminization. Wolbachia has close relationship with speciation and has great potential application in biocontrol.In this paper, the morphology of L. tricolor was observed with scanning electron microscope, this provided some basic data on the correct identification of the psocid and enriched the whole study of psocids. Then the infection of Wolbachia in L. tricolor was detected by molecular method, nad the structure and reproduction characteristics of Wolbachia was further observed with transmission electron microscope. The results of this paper will enrich the study Of Wolbachia and thus provid theoretic basis for pest control and the relationship between Wolbachia and its psocid host. The study was supported by the National Natural Science Foundation of China (30471173). Main findings are as follows:The external morphology of L. tricolor was observed with scanning electron microscope, the major characteristic feature were: The body length is 1.1mm and the body color is uneven. The head color is dark brown and the thorax is colorless, the abdomen is brown. This psocid was named as L. tricolor because of the three colors of its body. There were many fuzz and small tubercle in the calvaria, also there were scalelike configuration was divided by the small tubercle. There were 6 ommatidias on head and 8 sensillas on maxillary. The SI was short and the PNS was absence on the pronotum and there were 2-3 bristles on the notum and 4-5 bristles on the prosternal, besides, there were 6-8 bristles showing bow on the mesosternal. Then raised femur was observed. There was regulated bristles on the first and second segment of abdomen, and intersegmental membrane between the third and fourth segment. The tubercles on the abdomen nora was smaller than the tubercles which on the head, and the sculpture was like to the sculpture which on the head. Last, the bristles Mv9, Mv10 and Md10 were observed on the end of abdomen, also the bristle Se and D were observed. The T sclerite was inflated. The obvious differences were the body color and body length between L. tricolor and L. bostrychophila. The results of molecular detection showed that there was Wolbachia in L. tricolor, and a 605 bp fragment was amplified from genomic DNA of L. tricolor. The result of the BLAST comparison suggested that the sequence was the wsp gene of Wolbachia. The topology of the phylogenetic tree based on the wsp sequences showed that the Wolbachia in L. tricolor belonged to B subgroup.The inner structure of L. tricolor was observed with transmission electron microscope, and it was further confirmed that there was Wolbachia in L. tricolor. The Wolbachia in L. tricolor located in the cytoplasm near the yolk globule, meanwhile the follicle cell which has 3μm follicular cell nucleus inside was observed. The membrane of the nuclear was clear and there were mitcochondria and yolk globule in the cytoplasm of follicle cell. There was about 0.8μm empty vacuole near the mitcochondria. The Wolbachia with a cell wall surrounded and contain inclusion including spherical badies. The flagelliform bacteria and the reproductive mode like budding were observed. It was different with the binary fission, one end of flagellfform bacteria projected then formed a new ovoid bacteria which was different with parent cell.
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