Effects Of Exogenous Hormones On Ovarian Development And Contents Of Soluble Protein In Fat Body And Ovary Of Bradysia Odoriphaga

Bradysia odoriphaga Yang et Zhang is the main pest damaging the Liliaceae vegetables (such as leek, garlic, onion etc.), Asteraceae vegetables (such as lettuce) and edible fungi. At present, there have been no reports on the regulation of hormone in the vitellogenesis of this pest. This paper researched the regulation of hormones to ovarian development, the dynamics of soluble protein regulated by hormones and the effect of hormone inhibition on ovarian development. These results lay the basis of the regulation of hormones for vitellogensis of the insect, and the induction of hormones to vitellogenin gene.1 With different treatments of pyriproxyfen, molting hormone derived from plant and precocene II, we researched the regulation of different hormones for ovarian development of the pest. Pyriproxyfen has stimulus-reaction on the absorption of yolk proteins of ovary. Molting hormone derived from plant has no action and precocene II inhibits the synthesis of yolk proteins in fat body.2 The soluble protein in fat body, ovary and hemolymph of pupa, ovary of adult and egg were determined, the results showed that the contents of soluble protein in fat body peaked when the black pigment of small eyes of the pupa reached the bottem of the compound eyes, following the stage the contents of soluble protein lowed slowlly, and to the lowest in black pupa. The contents of soluble protein in ovary from white pupa to egg gradually increased, and peaked at egg stage. With ovarian development, the contents of soluble protein in hemolymph gradually declined.3 With different treatments of pyriproxyfen, molting homone derived from plant and precocene II, the effect of different hormones on the contents of soluble protein in ovary and fat body were researched. Pyriproxyfen can promote the contents of soluble protein in ovary and the decrease of the contents of soluble protein in fat body. Molting homone derived from plant has no action. Precocene II inhibits the raise of the contents of soluble protein in ovary and the decrease of the contents of soluble protein in fat body.