Differential MRNA Expression In Response To The Feeding Of Plutella Xylostella In Leaves Of Brassica Campestris

Plants employ a series of specific and unspecific responses with insect herbivory feeding. Although we thought highly of inducible defense reaction, the molecular response mechanism in vegetable induced by insect herbivory feeding has not been reported both at home and abroad. Differential display reverse transcription polymerase chain reaction (DDRT-PCR) method was used to isolate the differential expression genes in Brassica campestris in response to Plutella xylostella feeding. Fifty-seven differential display fragments were detected by siliver-staining denatured PAGE and nine positive clones were identified by reverse Northern dot-blotting. Through sequencing of partial positive clones, six clones containing only anchor primer sequences at both ends were identified. While one was verified as the PCR product of an anchor primer and random primer, and homology searches revealed that it was 99%(273/275) represented in the complete DNA clone of KBrH009B23 (GenBank, accession number AC 189564.1). This clone was also 68%(113/164) represented in the copine-related (AT5G14420) mRNA in Arabidopsis thaliana, and in plants, it could be related to the cell wall recovery according to the available research about the copine-related protein family.