| Haemaphysalis longicornis and the diseases that they transmitted are major economic burden to animal husbandry in China. Currently the principal tick control method is the application of acaricides. This approach is, however, associated with a number of disadvantages such as chemical pollution of the food chain and the environment as well as the quick development of resistance against acaricides by ticks. These limitations have necessitated the search for alternative tick control measures. Only anti-tick vaccinations among the several alternative tick control measures appear promising. For the search immunity of H. longicornis salivary gland protein , two sera were generated by different methods. A primary cDNA library was constructed in order to research the immune gene. The mRNA was cxtracted from salivary gland organs dissected from partially engorged H. longicornis. The cDNA was synthesized by reverse transcription and ligated to the arms of theλ-screen vector. The cDNA library was immunoscreened for obtaining functional antigen gene by western blotting. The ELISA test showed that the antibody in the infested group was positive after first infested in two weeks. The valence of antibody was rising along the increasing times of infesting and reached peak. The valence of antibody in the immune group continued to show an upward tendency in the eleventh weeks too. The resistibility effects of rabbits significantly were indicated in two groups. The library was a good quality one proved by all the control tests. The primary cDNA library was a size of 4.2×10~6 pfu and the amplification cDNA library was a size of 7.8×10~9 pfu/ml. Thus 26 positive genes were obtained. The size of those genes were among 199bp~1876bp after analysis of PCR, restriction digestion and sequencing. All those would be a good foundation for the development of anti-H. longicornis vaccine and for the clone of genes.
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