Identification Of Species And Genotypes Of Cryptosporidium Spp. Isolated From Different Species Animals In Shanghai District And Cloning And Expression Of CP15 And P23 Gene Of Cryptosporidium Paravum

Cryptosporidium spp. is a protozoan parasite and it is harmful severely to men and animals. It grows in epithelial cells and digestive organs of men, animals, birds, creeperes or fishes and it causes diarrhea. Cryptosporidium spp. is known to be ubiquitous and to have many hosts. The diarrhea can be cure by themselves about the normal immune people, but when people who had immune deficiency, the diarrhea maybe serious even take to death.There were different characteristics and hostes about Cryptosporidium spp. The species and genotypes of Cryptosporidium spp. isolated from mouse, rabbit, chicken, pig and dairy calves in Shanghai district were identified by nested polymerase chain reaction linked restriction fragment length polymorphism (PCR-RFLP). The results showed that there were three species and three genotypes, Cryptosporidium spp. which comes from mouse was Cryptosporidium paravum mouse genotype, rabbit was Cryptosporidium paravum rabbit genotype, chicken was Cryptosporidium baileyi, swine was Cryptosporidium paravum pig genotype II and dairy calves was Cryptospori—dium andersoni.The sporozoite surface antigen CP15 gene which came from C.paravum mouse genotype, C. paravum rabbit genotype, C. paravum pig genotype II and C. andersoni were successful amplified and encoded 119 amino acids. Cryptosporidium spp. came from different sources had a highly homoely and C. andersoni was first report in this expriment.The sporozoite surface antigen P23 gene which came from C. paravum mouse genotype, C. paravum rabbit genotype and C.paravum pig genotype II were successful amplified and encoded 113 amino acids. Cryptosporidium spp. came from different sources had a highly homoely. The prokaryotic expressing vector pET-28a(+)-CP15 and pET-28a(+)-P23 were constructed and expressed in E.coli successfuliy. Both of them had a well antigenicity which analyzed by ELISA.The development of our experiments we had done would much helpful to know the disease sources and epidemic conditions about Cryptosporidium spp. in Shanghai district, and had an important sense about public hygieology. The sporozoite surface antigen CP15 and P23 gene which were successful cloned might be much helpful for the next work of using these genes to develop a immunoprophylaxis or detection technique.