Detecting The Pharmacodynamic Action Of Artesunate And Ratinoic Acid And Establishing Of Real-time PCR Method For EH

Eperythrozoonosis is caused by the attachment of Eperythrozoon (EH) on the surfaceof erythrocyte with wild host range including human. EH was classified asanaplasmataceae, rickettsia. But based on the 16S rRNA analysis, it should bereclassified as Mollicutes, Mycoplasma. Such infection can induce fever, anemia andicterus and always coinfected with other pathogens. This diease had made a big loss inswine production. By now the common diagnostic method was microscopic exam. But itwas unreliable with high false positive rate. Ever used serum-diagnosis methods werecomprise of IHA, CFT, CIE, ELASA and IFA. But the fluctuated level of antibodyagainst EH infection accounted for the false negative results. Animal model assay wasdemonstated as good way for pathogen identification, howerer it was time consuming andinconvenient. Traditional PCR can only got the qualitative but not quantitiveresult. Therefor we established the real-time PCR method in this study.Based on the specific sequence of EH (accession number: AJ504999), a pair ofprimers was designed to amplify a 234bp fragment. And this band was comfirmed asEperythrozoon specific after sequencing.Artesunate and ratinoic acid were demonstated as the effective drug against EH infectionby studied in vitro and vivo assay including natural infected swine and experimental micemodel.