| Veterinary drug plays important roles in assuring the animal's health and improving its growth performance, but veterinary drug leads to the drug residues in animal when being misused. Meanwhile, they can go into body's ecosystem in the form of residues to endanger public health.The veterinary drug residues in food has become a serious and disputed problem since it is close to public health and has greatly affected the economic benefits of the animal husbandry. So it is a pressing problem to study the veterinary drug residues monitor. On the base of it, the paper studied the detection of Amprolium Hydrochloride(APL) in chicken muscle and the detection of Ethopabate(EPA), Amprolium Hydrochloride and Sulfaquinoxaline(SQ) mutil-residues in chicken muscle.1.The detection of the Amprolium Hydrochloride in chicken muscle chicken muscle was precipitated with acetonitril after homogenating , the acetonitril in the supenatant was extracted with n-hexane to eliminate fatiness and purified with waters oasis HLB. The mobile phase was uesed to resolve spent regenerant to determinate after eluting when using 5% methonal as solvent and acetonitril as spent regenerant after extraction column was activated. For the HPLC determination, an SunfireTM C18 column was used with a mixture of methonal and 5m mol/L sodium octanesulfonate solution which contained (2.4 mL/100 mL)acetic acid and triethylamine( 0.6 mL /100 mL) as mobile phase. The flow rate was 1 mL/min , the Uv detector was set at 270 nm and the column temperature was 30℃. The detection limit was 0.04μg/g for the Amprolium Hydrochloride. The stadard curve equation was Y=4.12e+004X﹣4.47e+002 and the correlation coefficient was 0.999907 when the linear range was 0.05μg/mL ~1.5μg/mL. The Amprolium Hydrochloride control solution was added to the blank chicken muscle to prepare three concentration chicken sample of 0.1μg/g, 0.2μg/g and 0.4μg/g, the average recovery was 82.292%±3.207%, the intra-assay precision was3.79%, 2.11% and 5.23% and the inter-assay precision was 4.74%, 3.13% and 1.43% respectively. These indexes all accorded with the residual detection demand. The result indacated the high-performance liquid chromatography(HPLC) method established was simple, fast, reliable and could be applied to the determination of chicken muscle.2.The mutil-residues detection of Ethopabate, Amprolium Hydrochloride and Sulfaquinoxaline in chicken muscle The sample was precipitated with acetonitril and the supenatant was extracted with n-hexane to eliminate fatiness , and the supenatant extracted was purified with waters oasis HLB. The 5 mmol/mL sodium dihydrogen phosphate was used as solvent and the methol as spent regenerant in the purying after the extraction column was activated,and the mobile phase was used to resolve spent regenerant after blowing with nitrogen to determinate. For the HPLC determination, an SunfireTM C18 column was used with a mixture of methonal and 5 m mol/L sodium octanesulfonate solution which contained (2.4 mL/100 mL)acetic acid and triethylamine( 0.6 mL /100 mL) as mobile phase. The flow rate was 1 mL/min , the Uv detector was set at 270 nm and the column temperature was 30℃. The detection limit of the three drugs was 0.05μg/g. The standard curve of the Amprolium Hydrochloride was Y=4.12e+004x﹣4.47e+002 (R=0.999907), the which of Sulfaquinoxaline was Y=7.77e+004x﹣1.76e+003 (R=0.999962) and the which of Ethopabate was Y=8.91e+004x﹣1.12e+003 (R=0.999967) when the linear range was 0.05μg/mL ~1.5μg/mL. The Amprolium Hydrochloride control solution was added to the blank chicken muscle to prepare three concentration chicken sample of 0.25μg/g, 0.5μg/g and 1.0μg/g, the average recovery was 78.358%±4.866%, the intra-assay precision was 2.970%, 0.425%and 6.934% and the inter-assay precision was 2.930%, 2.712% and 9.123% respectively. The Sulfaquinoxaline control solution was added to the blank chicken muscle to prepare three concentration chicken sample of 0.05μg/g, 0.1μg/g and 0.2μg/g, the average recovery was 73.291%±4.975%, the intra-assay precision was7.644%, 4.727% and 5.452% and the inter-assay precision was 10.468%, 4.124% and 6.528% respectively. The Ethopabate control solution was added to the blank chicken muscle to prepare three concentration chicken sample of 0.25μg/g, 0.5μg/g and 1.0μg/g, the average recovery was82.308%±2.416%, and the Intra-assay precision was 2.970%, 0.425% and 1.013% and the inter-assay precision was 5.823%, 4.551% and 14.155% respectively as well. The result indicated the chromatography method established could be applied to detect three drugs residual in the chicken muscle at one time.
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