The Influences Of Growth And Metabolism Of The Gametophytes Of Porphyra Haitanensis By Antibiotics

The Porphyra haitanensis is a nutritious and delicious alga. In the Europe and USA it is called Laver, and in the Japan it is named Nori. There are about 70 species of the Porphyra in the world. Now the main species of the artificial culture are P. yezoensis and P. haitanensis. The P. haitanensis is an important economic alga. So there are many researches to learn more about the alga. In the theses, the sdudy is focus on the effects of antibiotics on the growth and physiological processes of P. haitanensis.The study investigated the differences of growth and metabolism of the gametophytes of P. haitanensis treated with the four different antibiotics under different concentrations respectively by messuring the weight, the chlorophyll and proline contents. The culture condition for the gametophytes was 25℃, 2000lx, and 12:12h light:dark duration. The experimental results showed that the growth and metabolism of the gametophytes were not obviously affected by the amppicillin and gentamicin sulfate, when the concentrations of the two antibiotics were lower than 0.01mol/L. With increase of the concentration, interestingly, gentamicin sulfate promoted the growth of the gametophytesat at concentration of 0.01mol/L. The other two antibiotics, kanamycin and spectinomycin, obviously inhibited the growth and metabolism of the gametophytes when their concentrations were higher than 0.01mol/L. The inhibited effect of kanamycin was stronger then spectinomycin. So we might concluded that the amppicillin and gentamicin sulfate could be used for producting the aseptic gametophytes and the kanamycin and spectinomycin might be applied as selection pressure in the P. haitanensis genetic engineering.