Studies On The Antagonism Of Selenium To The Fluorine Reproductive Toxicosis In Cock

In this paper, 7-day-old cock (n=180) were used as experimental animals and certainconcentration of fluoride and selenium were added in the basic feed. Reproductive toxicologymodel caused by subchronic-fluorine toxicosis and antagonism model of selenium to fluorinetoxicosis were duplicated. The subchronic-fluorine toxicosis and antagonism of selenium tofluorine toxicosis were deeply studied through clinical symptom, pathological changes of testes,anti-oxidative function, apoptosis, the expression level of p53 and Caspase-3 mRNA in testes. Theresults showed as follows:1.The experimental results revealed that fluorine affected the anti-oxidative function of cock.Activity of SOD and GSH-Px decreased in serum and testes while the content of MDA increased,which constituated pathological basis of fluorine damaging the host body. The fact thatsupplementing selenium may decrease fluorine damage indicated selenium can antagonize thetoxicosis of fluorine to anti-oxidative system in cock.2.Fluorine decreased the content of T,FSH,E₂,GH and increased the content of LH in serum,which could be inhibited by selenium. This indicated selenium can improve the abnormal secretionof T,FSH,LH,E₂,GH. 3. The results of TUNEL and ultrastructure observation showed that fluorine toxicosis causedapoptosis in testes, adding selenium relieved the damaging effect. This indicated selenium canantagonize the apoptosis of testicular cell induced by fluorine.4. The microstructure and ultrastructure changes of testes demonstrated that fluorine couldmake the damage of mitochondrion, endoplasmic reticulum,nuclei and Blood-Testes Barrier ect,induce apoptosis or necrosis of leydig cells,support cells,spermatogenic cells. Selenium decreasedthe damaging effect of fluorine on organelles.5. The effect of flurine on DNA damage were detected by SCGE and DNA Ladder. The resultsshowed that fluorine can cause DNA damage of reproductive cells in cock and selenium candecrease the DNA damage caused by fluorine. Those results indicated selenium can antigonize thedamaging effect of testicular cells in cock caused by fluorine.6. The effect of fluorine on p53,Caspase-3mRNA expression was detected bysemi-quantitative PT-PCR. It revealed that fluorine can increase the mRNA expression of p53,Caspase-3 and furthermore induced apoptosis of reproductive cells. Selenium can antigonize theeffect of fluorine toxicosis by decrease the mRNA expression of p53,Caspase-3 and furthermoreantagonize apoptosis of testicular cells in cock caused by fluorine.Our study discussed the selenium antagonism to fluorine toxicosis in cock with celluar andmolecular view. Results revealed that selenium can antagonize the fluorine toxicosis whichpresente as decreasing the activation of germ cells, inducing oxidative stress, DNA damage,increasing apoptosis of testicular cells and changing the expression of apoptosis-related genes.Themechamisms of selenium antagonism to fluorine toxicosis in cock were illuminated. This reseachwas significant to offer the theory for protection and treatment of fluorine toxicosis in livestocks,enirch toxicology and Comparative Medicine. And, it is also meaningful to ensure the healthydevelopment of graziery, to protect wild fowl and to keep the ecological balance.