Pathogen Identification And Pathological Research On One Viral Disease In Goose

A large quantity of geese died in one place and a virus strain was isolated from thediseased geese, hemagglutination test showed that the virus could hemsgglutinatechicken's red blood cell; hemagglutination inhibition test showed that the virus wasinhibited by the standard Newcastle disease positive serum; toxicitical test showed thatMDT of the virus was 52.8 hours;molecular biology identification test showed that thevirus's sequence compared with other NDV strains,the homology of the nucleotide was96.0%～99.0%; experimental infection showed haemorrhage and ulceration of intestinemucosa, necrosis of spleen and pancreas in the diseased gostlings; resort to other studieson this virus strain, it was identified as one strain of the geese's paramyxovirus,andnamed GPMV/HB.The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT,B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet. Theconclusions of the goose paramyxovirus strain GPMV/HB experimental infection andpathological researches on diseased gostlings can provide important theoretical and theexperimental references to the researches of the geese's paramyxovirus.