Despration And Biocheracteristics Of Goose Newcastle Disease In A Goose Farm In YUNNAN Province

Typical goose Newcastle disease outbreaks in two goose farms in YunNan Province in 2007. The epidemiological results showed that the goose ND could occur in different ages geese and species, but the incidence rates of young and purebred goose were higher than that of adults and breeding. The incidence rates in the two goose farms were 100% and 80% respectively, and the mortality rates is 80%, 75% respectively.The samples of infected geese brain and spleen were collected, treated and inoculated 10-day-old SPF chick embryos. A ND virus (named strain KM2007) was isolated from the dead chick embryos. The isolate could agglutinate chick erythrocyte, and could be inhibited by the standard antiserum of the NDV, but it couldn't be inhibited by the standard antiserum of the AIV H5N1 and EDSV -76. It can be concluded that the isolate was goose ND virus.Twenty unimmunized chicken and geese of 25-day-old were inoculated with goose Newcastle disease virus strain KM2007 and the contrast group with physiological salt. As the result, all the tested chicken and geese showed typical clinical symptoms and pathological changes of ND and goose ND. It suggested that strain KM2007 is identical to the pathogen of natural goose ND.Ten-day-old unimmunized chicken and geese were inoculated with the inactivated oil-adjuvanted vaccine made from the isolated goose ND virus strain KM2007 and the contrast group with physiological salt. 21 days after vaccination, each chick and goose in test and contrast group was injected individually 0.1mL 10 diluted fluid of goose ND virus strain KM2007. As the result, chicken and geese in contrast group showed typical clinical symptoms and pathological changes of ND and goose ND. On the contrary, all the tested chicken and geese kept alive and healthy. Ten-day-old unimmunized chicken and geese were inoculated with the NDV inactivated oil-adjuvanted vaccine and the contrast group with physiological salt. 21 days after vaccination, each chick and goose in test and control group was injected individually 0.1mL 10-1 diluted fluid of goose ND virus strain KM2007. As the result, chicken and geese in contrast group showed typical clinical symptoms and pathological changes of ND and goose ND. On the contrary, all the tested chicken and geese kept alive and healthy.The tests indicated that both the inactivated oil-adjuvanted vaccines made from the isolated goose ND virus KM2007 strain and that from NDV can exempt chicken and geese from the attack of wild goose ND virus.