Effect Of Addition Antioxidant To Dilution On The Boar Semen Quality With Cryopreservation

In order to develop cryopreservation techniques of boar semen to be applied tocommercial production, and sieve optimum antioxidant added to boar semen extender,experiments were carried out to detect the activity of SOD,CAT,GPX,GR of boar sperm,and reveal the change during frozen-thawed process. V_C,V_E,SOD,CAT,GSH wasrespectively added to boar dilution, then the effect of the spermatozoa quality was estimateby detecting sperm motility,viability,plasma membrane integrity,acrosome integrity,ultrastruture and the content of MDA after cooling and thawing. The results were asfollows:There was an extremely significant lower post-thaw sperm motility, viability andplasma membrane integrity than fresh semen (P＜0.01). The activity of SOD, GPX, GRwere detectable, but none of CAT. After thawing, the activity of SOD and GPX werereduced, and the difference of SOD activity was extremely significant (P＜0.01). Thecontent of MDA was higher than that of fresh sperm and cooling sperm, and the differencewas extremely significant (P＜0.01). The correlation between the activity of SOD, GPX,plasma membrane integrity, acrosome integrity and the content of MDA was subtractive,but the difference was not significant (P＞0.05). The correlation between the motility,viability and the content of MDA was subtractive significantly (P＜0.01). The content ofMDA could be a index of estimating sperm quality.The addition of 10 mmol/ml V_C and 0.2 mg/ml V_E alone, increased post-thawedsperm motility, viability and protected Sperm plasma membrane significantly (P＜0.05),when increasing concentration the sperm quality reduced, but addition of V_C and V_Esimultaneity, the sperm quality did not increased; SOD and CAT alone added to freezing dilution improved post-thaw sperm motility, viability, plasma membrane integrity,acrosome integrity, especially the concentration with 100 U/ml of SOD and 200 U/ml ofCAT, addition of SOD and CAT in combination could improve sperm quality all the more.There was nearly no improvement by adding GSH in boar freezing dilution. Theexperiment observed the spermatozoon ultramicrostructure by electron microscope, the dilutioncombination with SOD and CAT could improve poss-thawed sperm quality.Therefore, addition of 10 mmol/ml V_C,0.2 mg/ml V_E, 100 U/ml SOD and 200 U/mlCAT or combination with SOD and CAT, could be used as a component in boarcryopreservation extenders, then it could provid a reference for cryopreservation technologyof boar semen.