The Determination Of Microelement And The Pigment Gene Mapping In Red Brown Rice

The pigment accumulates in pericarp and seed coat of red rice, with major studying ofspecial rice. Red rice, which is fragrant, daintily, special nutriment and health care effects,is planted and edible long in time in China. The effects contact with the pigment. It is oneof the ideal resources of health food. Therefore, the research is valuable for theory andapplication about the nutriment, health care effect, heredity analysis and gene mapping ofpigment.In this paper, we studied the histological analysis, physiological nature and eatingquality, to analysis the genetic action using the F₁ and F₂ progenies from red rice crossedwith common rice. Furthermore, the pigment gene of red brown rice was mapped withmicrosatellite markers. The results were as follows:1. The pigment was in pericarp and seed coat using paraffin section with the brownrice and glume. On the other hand, red was observed in the section of red rice glume. Itshowed the red pigment was in glume too.2. It was related to abundant of microelements to the nutrient and health care functionof red rice. Using plasma mass spectrograph and atomic absorption spectrometer todetermine the amounts of Fe, Zn, Se and Ge, the aim was to compare the nutrientdifference among the red rice, hybrid red rice and the common rice. The results were asfollows: (1)Fe and Ge was mainly in the seed coat and pericarp. (2)The red brown rice wasmore than common rice in the contents of every microelement and total contents of thefour microelements. The hybrid red brown rice was more than common rice too.(3)Crossing was an effective way to improve the yield and reduce the cost of health carerice because the nutrient of red rice was controlled by heredity. The red rice was potential resource of health care rice with high quality.3. GC and GT by chemical method, AAC by near infrared spectrum analyzer and ricestarch viscosity profiles by a Rapid Visco-analyzer(RVA) were investigated. Consideringthe four factors of eating quality, the study showed that R272 and R272/red rice B werebetter. So it can improve the eating quality of red rice by cross breeding.4. Genetic analysis and gene location were conducted on red rice. All grains ofreciprocal cross F₁ population derived from red rice and the white rice (R272) were all redrice, and the segregation ratio of the F₂ population was fitted to 3:1, showing that the redpigment trait was controlled by a dominant gene.5. The F₂ population of R272/red rice B was used as the mapping population. Thegenomic DNA of parent R272 and red rice were amplified with 507 pairs of primers ofsimple sequence markers well-distributed on 12 pairs of rice chromosomes. With theMAPMAKER using to linkage analysis, the pigment gene(temporarily named Red) waslocated between RM8006 (the genetic distance was 4.0cM) and RM21186 (the geneticdistance was 2.1cM) on the chromosome 7.