Study On The Viable But Nonculturable State Lactobacillus Lactis

The lactobacillus lactis is probiotics, mainly be applied in the new green feed additive which appearedin the recent years. It has the characteristic of the non-toxic side effect, non-residual, non-drug resistance,non-pollute-environment and so on. Its main function is supplementing microorganism in the quantityeither the type which the intestinal tract reduced ,to be restore microbiology balance of the intestinaltract,achieve the health care the goal. "The viable but nonculturable state"is a kind of special survival statewhen the bacterium is under the poor surroundings condition.The bacterium could not grow by normalcultured, but it is still viable and possesses metabolic activity.On the adequate condition,the bacteriumcould recover to culturable state. "VBNC" is short for it. The purpose of the experiment is researchingviable but non-culturable state lactobacillus lactis, take it into the research area of probiotics, find a kind ofmethod in producing new feed additive which prevents and treats illness.lactobacillus lactis R2 was offered by ShenYang Huishan Milk Co.Ltd, lactobacillus lactis R1,R3were separated from chicken and pig.Through researching lactobacillus lactis R1,R2,R3 and uniting themany kinds of detected way, in the end, the stability condition that the lactobacillus lactis was inducedinto VBNC was finded out, also the condition that viable but non-culturable state was resuscitation inlactobacillus has been explored. lactobacillus lactis was added into liquid MRS ,PH2-3,suspension, viable but nonculturable state wasinduced in lactobacillus lactis by 4℃low temperature. The time that lactobacillus lactis entered intoviable but non-culturable state was different, the shortest was R2,35 days, but the longest was R3,46 days.7% tween80 was added in viable but nonculturable state lactobacillus lactis suspension, which wasshocked at 37℃,200rpm, viable but nonculturable state lactobacillus lactis could become culturablestate.Tween80 is also a sort of familiar feed additive,which can add the amount of probiotics.Thecapability of feed additive is exerted more effectively. And normal R2 could resist strong acid, 0.2% bilesalt, and 1% pepsin. Contrastly, VBNC R2 could only resist strong acid and 1% pepsin except for bile salt.