Analysis Methods Of Carbendazim And Thiabendazole Residue In Edible Fungus

The use of pesticides and other agricultural chemical products made the environment and people very dangerous. For this reason the improvement of the analysis of pesticide residue is very obvious. For these years, the amount of the edible fungus products increases obviously. The analysis of pesticides residue in edible fungus is becomes a very important problem. Flammulina velutipes, Pleurotus ostracatus and Pholiota nameko are cultivated in every season all over the country. The research aimed to residual dynamic of Thiabendazole and carbendazim in the substrates of two kind of edible fungus cultivating in the factory to control the content of persistent pesticides in the substrates and make decision of reasonable using pesticides.In the paper, the detection methods of pesticides residue were established using HPLC method. The results indicate that this method is very right and accurate, the repeatability is good. The method was used to detect three fungicides in edible fungus and substrates. The results showed that linearity correlation coefficients of two pesticides residue were between0.9988 to 1, the recovery of carbendazim in substrates is 86.1%～87.9%, in Pleurotus ostracatus is 75.3%～84.4%, in Flammulina velutipes is 74.2%～89.4%. The recovery of thiabendazole in substrates is. 84.6%～87.5%, in Pleurotus ostraeatus is 81.9%～88.1%, in Flammulina velutipes is 82.4%～87.8%. Detection limitation amount of carbendazim was 0.001mg kg^-1, detection limitation amount of thiabendazole was 0.005mg kg^-1. All are conform to standard.The substrates samples were extracted by ethyl, and were cleaned up with Florisl. Then HPLC analysis was performed with Diamonsil C₁₈, eluted with methanol-water mixtures. The edible fungus was extracted by acetonitrile, and was cleaned up with Florisl. And the HPLC analysis is the same as the substrates. The degradation procedure was correspond to the mathematic pattern, C=C₀e^-kt. The dynamic equation of carbendazim in the Pleurotus ostracatus substrates is C=1.7253 e^-0.0819t. The dynamic equation of carbendazim in the Flammulina velutipes substrates is C=1.8709 e^-0.1177t. The dynamic equation of carbendazim in the Pholiota nameko is C=5.0308e^-0.439t. The dynamic equation of Thiabendazole in the Pholiota nameko is C=2.8189e^-0.384t. The half-life day of Carbendazim in the Pleurotus ostracatus substrates is t_1/2=2.97d and in the Flammulina velutipes is t_1/2=5.83d. The half-life day of Carbendazim in the Pholiota nameko is t_1/2=2.24d. The half-life day of Thiabendazole in the Pholiota nameko is t_1/2=1.05d.