Host Plant Preference Of Liriomyza Huidobrensis (Blanchard) On Different Phaseolus Vulgaris Varieties And Its Mechanism

The host plant preference of Liriomyza huidobrensis (Blanchard) was examined according to the number of stipples (ovipositing and/or feeding punctures) as a preference index for different Phaseolus vulgaris varieties and its relationship with the compound contents in P. vulgaris leaves. At the same time, the developmental duration of L. huidobrensis was tested.With no- choice method, the host preference of L.huidobrensis was examined for different Phaseolus vulgaris varieties.The results showed the preference of L. huidobrensis for different P. vulgaris varieties was significantly different. The clustering analysis separated the tested P. vulgaris varieties into three groups based on the levels of the preference of L. huidobrensis. The most favourite varieties included P. vulgaris cv. Shuangfengerhao, Futianyihaojiadou, Jingpinjiadou, Zihuayoudou, Dachengdidou, wujinglv, Shuangfengjiadou, Shijijiangdou, Tai-guojiadouwang, Taikongqingmei and Wuchangdayoudou. The second favourite varieties were P. vulgaris cv. Jiucunlian, Tianma95-33, Jingxuan73-8, Tianmadidou, Taifengjiadouwang, Futian'erhaojiadou, Manjialian, Taiguojiadou 95-33, Lvlongjiadou, Qiuzidou and Qingdaojiadou. The least favourite varieties included P. vulgaris cv. Junmadidou, Huajiangbahao, Tianmajialongwang, Chengpaijinglongwang and Xinxuanjiulibai.Acetone spectrophotometry, anthrone colorimetric method, Coomassie brilliant blue (CBB) G250 staining, phosphor-molybdenum acid-phosphor-tungstenic acid colorimetry and Soxhlet extraction were applied to measure the contents of lamina's chlorophyll, soluble sugar, soluble protein, tannin acid and flavone, respectively. Chemical component analysis showed that the contents of chlorophyll, protein, soluble sugar, tannic acid and flavone were significantly different among the tested varieties. Correlation analysis showed that the host preference of L. huidobrersis was significantly and negatively correlated with the contents of tannic acid and flavone, but had no significant correlation with the contents of chlorophyll, soluble protein and soluble sugar in the varieties.The developmental duration of L.huidobrensis was tested on 6 representative varieties. The results showed the developmental durations of the egg,pupa and larva in different P. vulgaris varieties were significantly different. The pupation rate of the larva was significantly different, but the emergence rate was not different.