Isolation Of Activity Yeast And Its Application In Weaned Piglets Dietary

Strong resistance Saccharomyces cerevisiae were isolated from piglets feces andtheir probiotics roles were investigated by in vitro experiments and in vivo in piglets.The results were described in the following three sections.SectionⅠ: 63 strains of yeast were isolated from piglets feces through the acidand bile salt resistance experiments, high copper,high zinc resistance experiments andtheir antibacterial capacity to major pathogenic Escherichia coli serotype K88, K99and 987P were investigated by antibacterial tests. Saccharomyces cerevisiae S19 isthe best one and its Antibacterial distances to K88, K99 and 987P are 2.1, 1.6 and1.4cm.SectionⅡ: Experiment were conducted to evaluate the effects of live yeastsupplementation on nursery pig growth performance, intestinal microflora and villuslength to determine whether live yeast could replace antibiotics and its bestsupplementation level. 180 piglets were weaned at 21 d of age (BW=7.56±0.51 kg)and assigned randomly by weight to 5 groups (treatments, six pigs per pen with sixpens per treatment). Diets type: (1) based diet (2) based diet+10mg/kg Flavomycin,(3)diet+0.05% Saccharomyces cerevisiae, (4)based diets+0.10% Saccharomycescerevisiae (5)diet+0.15% Saccharomyces cerevisiae.The following were the results ofthe experiment: The group added active yeast increased ADG in second phase(P＜0.05),but did not see significantly different in final weight(P＞0.05) than thecontrol group. Saccharomyces cerevisiae can significantly increase the intestinalyeast and lactic acid bacteria, and decrease the number of E. coli; Meanwhile,increased intestinal villus height. The addition of 0.1% is the best.SectionⅢ: 72 piglets were weaned at 21 d of age (BW=7.05±0.0.47kg) wereassigned randomly by weight to 3 groups (treatments, six pigs per pen with four pensper treatment) to investigate the impact of high copper(225mg/kg),high zinc(2250mg/kg) to Saccharomyces cerevisiae probiotics role. Three diets were baseddiets, based diet+10mg/kg Flavomycin, based diet+0.1% yeast. Research shows that at the dose of Cu,Zn in the experiment, there was no antagonism between Cu,Zn andSaccharomyces cerevisiae.