The Study On Zearalenone Induced Apoptosis Of Mouse Granulosa Cells And Correlation Influence Factor

Zearalenone(ZEA), i.e.F-2 toxin, is a mycotoxin produced by Fusarium species. Itmainly empoison feed and cropper, such as wheat oat corn and so forth.Zearalenonehas strong estrogenic activity associated with hyperestrogenism and physiologicalalteration of the reproductive tract. In addition, ZEA still has the genetoxic,immunity toxicity and latent cancer-causing, to the threat of the animal and mankind's health very huge. So we have the research on toxicity of ZEA to enhances animal'sreproduction performance and improves the livestock product quality.Research Subject: discuss the toxicity and fuction mechanism of F-2 toxin on theovary granulose cells. The theory of ZEA-induced the toxicity on gonad was renewed.The theoretic evidence was provided for later study.Research methods: Prepubertal female mice as object injected with several doseF-2 after subcutaneous injected with PMSG.The apoptosis of granulosa cells weredetected by TUNEL; the content of MDA and the activeness of SOD were detected byconventional method; the p53 protein expression was detected by immunityhistochemistry and the semi-quantitative RT-PCR.Research Results: The TUNEL result showed that the apoptosis percent ofgranulose cells in high, midst and low-dose groups are 8.4635%, 8.9634%, 11.7563%respectively after 48 hours, which are higher than control groupsignificantly(0.6402%, P＜0.01). The apoptosis percent in control, high, midst andlow-dose groups grow up to10.9832%,11.2134%,12.0231%,18.8437%respectively after 96 hours. The result showed that the apoptosis in diverse degreewere occurred in every groups, the apoptosis index increase with dosage rising, thereis extreme significant difference (P＜0.01). The percent of atresic follicular increasedwith dosage rising (P＜0.05). To examine the content of MDA and the activeness ofSOD by conventional method, the content of MDA increased with dosage rising, thereis extreme significant difference (P＜0.01) between testing group and control groupin the two time section of 48h and 96h. The activeness of SOD decreased with dosagerising, there is significant difference between control group and testing group on 48 hours, there are extreme significant difference (P＜0.01) between control group andtesting group on 96 hours. The result of expression of immunity histochemistry andthe semi-quantitative RT-PCR examining p53 protein expression showed that p53express significant difference between testing group and control group (P＜0.01).Research Conclusion:1. Zearalenone can accelerate the apoptosis of granulose cells and is related with dose.2. Zearalenone can increase the content of MDA of granulose cells and can decreasethe activeness of SOD.3. The p53 gene participated in the regulation of the apoptosis of granulose cells,Zearalenone can enhance the expression of p53 protein in granulose cells.4. Apoptosis of granulose cells induced by Zearalenone is the result of free radical andregulative gene affected together...