Research Of The Best Cell Culture Condition And White Rats' Immunogenecity On Proplis Inactivated GE～-/gI～- PRV Vaccine

The experiment mainly study pig pseudorabies virus gene deficency(PRV gE~-/gl~-) and PRV gE~-/gl~-made by Professor SHENZhiQiang from shandongprovincee, respecting to theoretical testimony for application of thevaccine. The results are as follows:Firstly, we Separate the development to propagate the PRV gE/gI withthe Vero, compare the vaccine stub in the adaptability on each cell suchas Marc145, BHK21, PK15 and CEF etc, and saw the Cytopathogenic effect.As the same time, we had compare the vaccine stub on each cell of poisonousprice and so on, and choosed the cell to carry on the next experiment.Secondly, we carried on the development of the vaccine stubconditional and excellent turn the research, in order to make to have theantigen to provide to experiment the data reference in great quantities,to attain to use the most low cost production but acquire the ideal andhigh antigen quantity.Thirdly, we carried on the biology characteristic research of thevaccine stub, examine the biology characteristic of the vaccine stub,provide the biology theories foundation fully for the vaccine system.Fourthly, According to the three-R, application the method of theimmunology, take small white rat of the experiment animal as the animalmodel, carried on the research to the cell immunity aspect, The cellimmunity aspect, measure the spleen heavy, the whole body is heavy,computing a spleen heavy of weight ratio;Use the MTT method to the lymphoidcell of spleen while stimulate the original function of propagate theability to carry on the measurement, reflect the cell immunitycircumstance with the indirectly. As a result express that the propolisvaccine expresses an antibody creation well. The spleen heavy of theweight ratio certificate propolis vaccine to the machine body immunityshould the answer promote the function, ln addition, through pour to turn to experiment the confirmation, the propolis vaccine immunity canincrease the lymphoid cell of spleen to stimulate the original functionunder of propagate the ability.On all accounts, the propolis vaccine can improve the cell immunityability of the machine body, The keyword pig pseudorabies geneimperfection vaccine virus stub antigen system has the immunity originalof the vaccine stub biology characteristic vaccine. It's a kind of worthyof universal expansion of new vaccine.