| Cell suspension culture of a Chinese herbal plant, Polygonum cuspidatum Sieb. et Z. was established in this study. Key factors including medium formulation, culture conditions and elicitors were investigated for optimal cell growth and secondary metabolite production. Effects of explants types on the metabolic capacity of in vitro cell cultures were also tested.Cell suspension cultures of P.cuspidatum were firstly established from stem explants-derived callus in MS medium supplemented with 0.5mg/L 2,4-D, 0.8mg/L 6-BA and 30 g/L sucrose at 100rpm, 25±2 oC and light intensity of 300μEm-1S-1 with a photoperiod of 14h/day. In a kinetics study of 20 days cell culture, the maximum biomass was obtained on day 7. The production of both total phenolics and flavones was uncoupled with cell growth.To optimize the cell growth and metabolite production, the effect of phytohormone combinations, basal medium, inoculum density, sucrose concentration and photoperiod was studied. The optimal medium formulation was found to be 1/2MS + 0.25mg/L NAA + 0.5mg/L KT + 30 g/L sucrose. Among four different elicitors tested, Jasmonic acid (JA) at 20μmol/L was proved to be the best to maximize total phenolics and flavones production. When 20μmol/L JA was used in combination with the optimal medium formulation, the yields of total phenolics and flavones were increased by 11 and 21 times, respectively.It is unfortunate that the cell suspension culture established from the stem explants did not produce one of the major bioactive compounds from this herbal plant, stilbene...
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