Study On The Pleurotus Cornucopiae Protoplasts Technology

The conditions of separation and regeneration of Pleurotuscornucopiae protoplasts were studied. The results showed the optimal forPleurotus cornucopiae protoplast separation were that the age of hyphe,lywallzyrne consistency and stabilizer was 3 days, 2% and 0.6mol/L MgSO₄respectively. The Pleurotus cornucopiae the age of hyphe was.1 day, thedigesting time was 1 hour, the osmotic stabilizer was 0.6mol/L scourer, thefrequency of regeneration was the best and reach up 4.5%.The conditions of PEG fusion between Pleurotus cornucopiaeprotoplasts and Pleurotus ostreatus protoplasts were studied. The resultsshowed the optimal PEG concentration, Ca²⁺ concentration, temperature,treatment time, and PH value was 35%, 0.010mol/L, 20mintius and 8respectively, the frequency was 3.3×10^-4.The conditions electro fusion between Pleurotus cornucopiae protoplastsand Pleurotus ostreatus protoplasts were studied. The results showed optimalAC frequency, AC strength, DC pulse strength of, DC pulse PW, number of DCpulse number of, mannitol density and CaCl₂ density was 1500KHz,300V/cm, 2.5KV/cm,60μs, 4～5times, 10% and 0.4mmol/L respectively, thefrequency was 21%.