Study On The Effect Of A. Japonica (Thumb) Miq.and Bacillus Sp. On Immune Function And Intestinal Microbial Population In Mice

Object: In order to explore the effect of association of A.japonica(Thumb) Miq.and the praperation of Bacillus sp.on immune function and performance trait as feeding additives. Used mouse as a model and evaluated the effect on immunity function and intestinal microbial population.Methods: A.japonica (Thumb) Miq.aqueous extractive and alcohol extractive were prepared to determine bacteriostasis activity of A. japonica (Thumb) Miq.against Bacillus sp. by using Tibe-Dish and Test-tube and geometric-progression diluting methods. Kunming species healthy male mice were treated with Oxybicycline (At), A.japonica (Thumb) Miq. (A). Bacillus cereus (Bc), Bacillus subtilis (Bs) and the mixture of A+Bc and A+Bs. The 140 mice were divided into seven groups randomly: Control group (C) was fed the basic diet, Bc group was fed the diet with 2.0×10~6cfu/g of Bacillus cereus additive, Bs group mice were fed the diet with 2.0×10~6cfu/g of Bacillus subtilis additive, A group was fed the diet with 2% A.japonica(Thumb)Miq, A+Bc group and A+Bs group were fed the diet with same dose of above additive, At group was fed the die with 1‰Oxybicycline as positive group. At the 10th, 20th, 30th day, average weight gain was determined on an empty stomach before feeding. Histological section of immune organ was used to investgate the change of histology among study groups. At the 30th day, blood from the mice of all groups were collected and the serum was separated to measure the enzymatic activity of blood serum lysozyme, and the spleen cells were collected to perform the proliferation capacity after stimulation by ConA. At the 30th day, ileum contents were adopted to detect the intestinal microbial population.Results:(1) Alpinia japonica aqueous extractive and alcohol extractive had no bacteriostatic efficacy on the growth of Bacillus sp. in vitro.(2) The changes of weight gain of the group A,Ac and As were much higher than the group C and At (p＜0.01). The other groups were quiet (p＞0.05).(3) The T cell proliferation capacity of the group Bc and Bs were much more enhanced compared to that of the group C and At (p＜0.01), and significantly higher than the group A,Ac and As (p＜0.05). The serum level of blood serum lysozyme had the same results.(4) Comparied with the group C and At, the contents of Bifidobacterium and Lactibacillus were significantly higher (p＜0.01), and Enterococcus was lower (p＜0.05). The contents of Enteric bacilli of the group At was much higher than any group (p＜0.01).(5) The spleen of the group Bc and Bs was observed under the microscop, which white pulp district was enlarged and the amound was increased and germinal cente was seen obviously. The cell density of cortex and medulla of thymus was much higher in the group Bc and Bs, and that of the group At was lower than others.Conclusion:(1) Alpinia japonica aqueous extractive and alcohol extractive have no bacteriostatic efficacy on Bacillus in vitro.(2) Bacillus sp. could promote the growth of mice's immune organs such as spleen and thymus, stimulate the cell multiplication of splenic lymphocyte and increase the serum level of lysozyme, which could regulate and strengthen the mice's immune function.(3) A.japonica (Thumb) Miq. can significantly increase the mice's weight gain, but have less effect on the mice's immune function.(4) A.japonica (Thumb) Miq. and Bacillus sp. can obviously promote development of intestines, profit to the permanent planting of intestinal normal microbial population and regulate microecological balance, which has definite reinforcement action on the mice's immune function.