The Study On The Toxicity Of Deltamethrin On Carassius Auratus And The Residue Detection

This paper studied the comprehensive evaluation of deltamethrin pesticides for give warranty of its safely use in aquiculture. It was studied the acute toxicity of deltamethrin on Carassius auratus. It discussed the harm of deltamethrin to detoxifcation mechanism in Carassius auratus by detecting the effect of deltamethrin to alexipharmic metabolism-enzymes in different tissue. The effect of deltamethrin to target enzymes(AChE) was studied to explore its harm to physiology functions. The genetic toxicity of deltamethrin to Carassius auratus was studied too. Gas chromatography wih electron capture detection method was developed for determining the residues of cypermethrin, fenvalerate and deltamethrin in fish. The mainly results were summarized as follows.The results shows that under testing controlled conditions the median lethal dose at 96h (96h-LC₅₀ value of deltamethrin on Carassius auratus was 61.17pg/L, and the safe concentration was 6.117μg/L. Deltamethrin pesticides is high poisonous to Carassius auratus.Under testing controlled conditions, the alexipharmic metabolism-enzymes in different tissues approximately were induced in Group2.039μg/L-Group6. 117μg/L, deltamethrin didn't hurt the antioxidant function. In different tissues, the change of the activity of alexipharmic metabolism-enzymes were not uniform, the change in gill was more prominent than in liver and kidney. It was also found that solvent would influence the toxicity of deltamethrin.Under testing controlled conditions, the activity of AChE in different tissues were approximately restrained firstly and induced later in Group2.039μg/L-Group6. 117μg/L, deltamethrin didn't hurt the physiology functions. In different tissues, the change of the activity of AChE were not uniform, the change in liver was more prominent than in gill and kidney. It was also found that solvent would influence the toxicity of deltamethrin, which was accord with the results of last experimentation.The Group2.039μg/L-Group4.078μg/L were not distinctly diversity with Contrast Groups when the genetic toxicity of deltamethrin to Carassius auratus was studied. The micronucleus frequency and nuclear anomalies frequency of Group6.117μg/L were changed distinctly and the laws were conform. Increasing the concentration and exposure time, increased the micronucleus frequency and nuclear anomalies frequency. Deltamethrin was genetic toxic to Carassius auratus.The sample were extracted by acetonitrile, defatted with hexane, then passed through ENVI-18 column and AL-N column. The samples were ready for determination. Results showed that the new method has good linearity and repeatability which the quality concentration of cypermethrin, fenvalerate and deltamethrin were 1.0μg/L-500.0μg/L. The recoveries were 78.5%-100.8% which the spiked concentratios were 1.0μg/Kg-10.0μg/Kg. The relative standard deviations were 3.1%-9.4% (n=5). All the detection limits of 3 pyrethroids were 0.2μg/Kg. The method is applicable for detection ofpyrethroids residues in fish.