The Establishment Of Particle Bombardment Transformation System For Aluminum Sensitive Wheat

Wheat (Triticum aestivum L.) is one of the most important crops in the world. In recent years, the development of the industry has led to over activation of Al in the soil, which greatly restricts the wheat growth and output. Therefore, it is very important to improve wheat quality and Al resistance by plant genetic engineering.To establish particle bombardment transformation system for aluminum sensitive wheat, the plasmid transformation and large-scale preparation, the effect of different concentration of Aluminum on induction and differentiation of wheat mature embryo and the effect of kanamycin on the contamination of wheat embryo were studied. Furthermore, the effect of pre-culture of immature embryo before bombardment on GUS transient expression was also discussed.The result of the experiment indicated that the plasmid transformation frequency was improved by TB disposal, the induction and differentiation rate of EM12 mature embryo were affected more remarkably than that of Atlas66, the contamination rate of wheat embryo dropped during the induction with the increase of kanamycin concentration, but the induction rate was not related to kanamycin concentration. Moreover, the different pre-culture time of immature embryo greatly influenced the GUS transient expression. GUS expression was higher when induced for 10 days than those induced for 4 days or 24 days. The GUS activity was the highest when Mannitol concentration was 0.75mol/L, and the blue stain number was 4-5 times of the control.