Studies On Chinese Herbal Medicinal Pigmentation Feed Additive For Sanhuang Broiler

Sanhuang broiler is a local breed of China. Its yellow feet, skin and beak are liked by consumers. But it is difficult to reach the desirable color through feeding of ordinary feed. The feed must be added with pigment. Nowadays chemosynthesis pigment is used mainly. Along with the problem of food safety is concerned, the application of chemosynthesis pigment is limited. Many Chinese herbal medicines contain abundant pigment and have many advantages, such as extensive source, simple preparation and little side-effect and so on as feed additive. This research observed the pigmentation effect of Chinese herbal medicinal feed additive for Sanhuang broiler by filtration of principal drug, Chinese herbal medicinal prescription and the prescription dose and security determination of the prescription respectively in order to develop a Chinese herbal medicinal pigmentation feed additive for Sanhuang broiler. In the same times the efficacy of the prescription on layer was observed to probe into the possibility for layer.Test 1. Filtration of principal drug 175 10-day-old sanhuang broilers were randomly divided into 7 groups. The chickens in 6 test groups were fed with feedstuff respectively containing the powder of 0.5% capsicum, 1% gardenia fruit, 5% green tangerine peel, 5% sweet wormwood, 0.5% CHM A flower and 0.5% CHM B flower, and the group of black control were fed with basic diet. Their color of foot skin was compared. The results showed that group CHM B presented the best color increasing effect. 75 10-day-old sanhuang broilers were divided randomly into 3 groups. The chickens in Chinese herbal medicine group and in chemical pigment group were fed with diet contained 0.5% CHM B powder and 30 mg/kg carophyll yellow and 10 mg/kg carphyll red , and the group of black control were fed with basic diet respectively. Their color of foot, chest and neck part skin was compared. The results indicated that the skin colors of group CHMB was markedly higher than chemical pigment and blank control group(P < 0.05).Test 2. Filtration of Chinese herbal medicinal prescription The CHMB was main and composed 3 compounds Chinese herbal medicines. In experiment, 150 10-day-old sanhuang broilers were randomly divided into 5 groups. The chickens in 4 test groups were fed with feedstuff respectively containing the powder of 1% compounds Chinese herbal medicines 1, 1% compounds Chinese herbal medicines 2, 1% compounds Chinese herbal medicines 3 and 30 mg/kg carophyll yellow and 10 mg/kg carphyll red .Group 5 was black control fed with basal diet. The test was carried for 4 successive weeks after adaptive phase of 4 days. Their color of foot, chest and neck part skin was compared. The results indicated that the skin colors in all of compounds Chinese herbal medicines groups were markedly higher than those in chemical pigment and blank control group(P < 0.05), and compounds Chinese herbal medicines 3 presented the best color-increasing effect.Test 3. Filtration of the prescription dose The compounds Chinese herbal medicines 3 was disparted low, middle and high doses. 150 10-day-old sanhuang broilers were randomly divided into 5 groups. The chickens in control group were fed with basal diet. The chickens in the 3 dose groups of Chinese herbal medicine were fed with the feedstuff respectively containing 0.5%, 1% and 2% compound Chinese herbal medicine, in chemical pigment group, containing 30 mg/kg of carophyll yellow and 10 mg/kg of carphyll red. The test was carried for 4 successive weeks after adaptive period of 4 days. The color of foot and chest skin was compared. The results indicated that compound Chinese herbal medicine at high and middle doses could markedly increase the colors of foot and breast skin(P < 0.05), without significant difference between high and middle dose group.Tset 4. Determination of the prescription security The best dose compounds Chinese herbal medicines in broiler and laying hens was received Experimentation of Security. Acute toxicity test: 60 small white mices were randomly divided into 3 groups. The mices in control group were poured with 0.5 mL physiological brine by stomach with 1 times one day. The mices in the test groups were poured with 0.25 mL and 0.5 mL decoction fluid of Chinese herbal medicine respectively with 1 times one day, which last 3 days. The results indicated that the mice were well in acute toxicity test. Sub-acute toxicity test: 63 laying hens were randomly divided into 3 groups. The hens in control group were were fed with basal diet. The hens in the groups of Chinese herbal medicine were fed with the feedstuff respectively containing 1% and 2% compound Chinese herbal medicine 3, which last 90 days. The laying rate, average egg weight, health status, avoirdupois, encyme of liver, change of anatomization and pathology were observed. The results indicated that the hens were kept higher laying rate and average egg weight and normal encyme of liver, change of anatomization and pathology.Test 5. Efficacy of the prescription using in layer The best dose compounds Chinese herbal medicines in broiler was used to laying hens. 23-week-old 63 laying hens were randomly divided into 3 groups. The hens in control group were fed with basal diet. The hens in the groups of Chinese herbal medicine were fed with the feedstuff respectively containing 1% compound Chinese herbal medicine, in chemical pigment group, containing 30 mg/kg of carophyll yellow and 10 mg/kg of carphyll red. The test was carried for 30d. The pigmentation grade and carotenoids content in yolk were measured. The results indicated that compared with group of chemic pigment and blank control the compound Chinese herbal medicine could markedly increase the colors of yolk and carotenoids content in yolk (P < 0.05).