The Study On Intestinal Histology And Developing Regulation Of Nutrients Of Alogynogenetic Crucian

In order to provide scientific evidence for the farther study on digestive physiology in carps, the intestinal histology, morphology and G cells distributive density in Allogynogenetic crucian was observed firstly, and then the intestine was compartmentalized according to the observational results; secondly, the intestinal histology and morphology varieties of Allogynogenetic crucian in different developing phases were studied; at last, the effects of cottonseed meal hydrolysates and Soybean meal hydrolysates on the growth of Allogynogenetic crucian and the mechanism were discussed. This study consists of three parts as follows:1. The observation on histology and morphology of the intestine and the study on the intestinal function in Allogynogenetic crucianAllogynogenetic crucian was researched in this experiment whose intestinal histology and Gastrin positive reactive cells' distributions in the gut mucosa were examined through paraffin slice and immunohistochemistry with the purpose to study each intestinal part's function in fishes without stomach. The results indicated that there were evident varieties in the sizes of the tunnels, in the thicknesses of musculature, and even in the duplicatures' numbers, heights, and widths; among them the size of the tunnel and duplicatures' number decreased gently from the foreside to the end; Gastrin positive reactive cells could be found everywhere in the mucosa along the whole gut of Allogynogenetic crucian except the part which was close to the anus, and the cells' distributive density decreased along the gut from the foreside to the end. According to the results, the gut can be compartmentalized into fore-gut, mid-gut, hind-gut and rectum; the percentages of each part to the whole length are 13.44%±2.19%, 39.42%±0.70%, 38.64%±1.94% and 8.50%±1.02%. 2. The study on the intestinal histology and morphology varieties in different developing phases of Allogynogenetic crucianThe foregut, midgut and hindgut's intestinal histology and morphology in 3 develop periods (prophase-young, anaphase-young and adult) of Allogynogenetic crucian were observed, with the purpose studying the varying characters of the intestinal histology and morphology in different develop periods. The results showed as follows: the varying trends of duplicature's number, duplicature's width, musculature's thickness and tunnel's size had been the same respectively in 3 develop periods; among them, duplicatures' numbers increased from foregut to midgut and decreased down to hindgut all the time in 3 develop periods; duplicature's width in foregut were still bigger than the other parts of the intestine all the time, and there was no difference between the midgut and the hindgut (P>0.05); both musculature's thickness and tunnel's size decreased from foregut to hindgut all in 3 develop periods; but the varying trends of duplicature's height were changed in 3 develop periods; the varying trends in anaphase-young increased from the foregut to the midgut and decreased down to the hindgt while the varying trends in prophase-young and adult both decreasing from the foreside to the end.3. The study on plant protein hydrolysates' effects on the intestinal development of Allogynogenetic crucian.The effects of the cottonseed meal hydrolysates and Soybean meal hydrolysates on growth performance were investigated in Allogynogenetic crucian through paraffin slice and immunohistochemistry in order to study its action mechanism. 360 fishes (initial weight ca.35.05g) were used in this trail. Experiment fishes were divided into three groups randomly and the fishes in the same group were fed in three aquatic cases (40 fishes/ case). Group I were fed with basic diet; group II and group III were fed with the basic diet with 3% cottonseed meal hydrolysates and 3% soybean meal hydrolysates in stand of 3% cottonseed meal and 3% soybean meal respectively. Period of this trail was 120 days. The results showed as follows: the intestine length in group II and group III had been longer, but were not different to group I (P>0.05); cross section area in group II and group III were bigger significantly than the one in group I (P<0.01); intestinal histology and morphology varied, contrast to group I , duplicature's numbers, duplicature's heights, duplicature's height/ musculature's thicknesses and intestinal mucous membrane's areas of group II and group III were both increased, duplicature's numbers were different(P<0.05), others were significantly (P<0.01); the G cells distributive density of group II and group III had also increased, group II was significantly different (P<0.05), but group HI had no difference (P>0.05).