Isolation And Identification Of Duck-Source APMV-1 And Preparation And Immunity Effect Observation Of Duck-Source APMV-1 Inactivate Vaccine

From September, 2005 to March, 2006, a violently infectious disease spread in duck groups in ZhouZhi, Shannxi Province, which resulted in severe jeopardize to the poultry industry of this area and caused significant economic loss. In order to control the spread of this disease, we have done the following researches.1. Two strains of virus, ZH1 and ZH2, were isolated from the infected duck's livers by egg inoculation method. The two strains of virus could agglutinate the erythrocytes of chicken,duck,sheep,goat,pig,human,rabbit,cattle and the other animal species. The character of hemagglutination of the virus could be inhibited by the antiserum of Newcastle disease virus (NDV).According to the international standard and method of virulence judgement of NDV, we measured the average death time(MDT),the minimal lethal dose(MLD) and the median infective dose(EID50) of chicken embryos and the intracellular inoculation pathopoiesis index of 1-day-old chicks (ICPI). We determinated that the MDT of ZH1 and ZH2 were 52h and 44h respectively, the EID50 were 106.4/ 0.1mL of ZH1, and 108.64/ 0.1mL of the ZH2, the ICPI were 1.930 of ZH1, and 1.975 of ZH2. The results indicated that the two isolated strains of virus were velogenic strains, and it is affirmed that they were Newcastle disease virus.2. In order to evaluate the immunity effect of NDV inactivated vaccine to Cherry Gu duck, we inoculated the duck-source NDV which was isolated from duck into the 9-10-days-old chicken embryo, and collected allantoic fluid of the chicken embryo, then added the inactivator, and made it into Oil-adjuvant inactive vaccine and water-adjuvant inactive vaccine. Inoculate 15-date duck with the duck-source NDV Oil-adjuvant inactive vaccine, water-adjuvant inactive vaccine and NDV LaSota hypotoxicitious vaccine respectively, then collect blood after 0, 7, 14, 21 days since inoculating, and measure the HI valence. After 21 days, we infected duck with the duck-source NDV allantoic fluid we had isolated, we got the protect rate: 100% to the oil-adjuvant inactive vaccine; 77% to the water-adjuvant inactive vaccine; NDV LaSota hypotoxicitious vaccine was 0; the duck of control group were all morbility and died. The results of this test suggested that there may be exist antigen difference between the duck-source NDV and traditional chicken-source NDV.616.9...