Study On In Vitro Regeneration And Variation Induction Of Actindia

In this study, the endosperm culture and colchicines treatment were conducted to investigate the mutational induction of different material from Actindia.1. An endosperm plant regeneration protocol for Actindia were achieved via organogenesis from callus tissues derived from endosperm explants both of Actindia chinensis cv. 'Jintao' and Actinidia deliciasa cv. 'Hayward', in vitro. In this paper, the effects of different combinations of plant growth regulators, sucrose concentrations, medium formations and dark treatment on callus induction and shoot formation were examined. The addition of 2,4-D increased percentage of endosperm explants forming calli; the maximum callus induction frequency were observed in the half-strength MS medium containing 1 mg/L 2,4-D, 3 mg/L BA and 20g/L sucrose. After inoculated in dark for 7days, the maximum shoot regeneration frequency was observed in half-strength MS medium containing 0.1μmol/L NAA, 5μmol/L BA and 20g/L sucrose. The differentiation ability of callus derived from 'Jintao' used as explant was higher than the 'Hayward'.2. This paper described a colchicines-mediated technique for the in vitro ploidy induction of different callus of Actinidia. The shoot regeneration frequency and shoot mutational rate were observed. The difference was significant observed in the shoot regeneration frequency and shoot mutational rate after colchicines treatment. The leaves characteristics were sharply changed, the width was reduced. Simultaneously, the internode length of shoot was reduced.3. This paper describes a colchicines-mediated technique for the in vitro ploidy induction of seeds of Actindia chinensis cv. 'Jintao'. The seedling emergence rate and mutational rate were observed in different concentrations of colchicines solution and different treatment time. The result shows that the seedlings of 'Jintao' . were shown variation some extent. The height, length and width of leaf were reduced. One dwarf mutant was gotten by soaking seeds for 24 hours with 0.20% colchicines solution.