Study On Reproductive Technology Of Actinidia Arguta

In this paper,the variety of Actinidia arguta "Longcheng No.2" was used as the test material to study various rapid propagation methods of Actinidia arguta.Methods such as cutting seedlings and tissue culture seedlings can be used to obtain a large number of seedlings in a short period of time,and a rapid propagation system suitable for Actinidia argutat can be established;the breeding characteristics and pollination ecology of Actinidia argutat can be studied to ensure the success rate of pollination.The main conclusions are as follows:(1)During sowing and propagation,the seeds were sterilized by 75% ethanol for 5minutes and then soaked in 2% sodium hypochlorite solution for 10 minutes,and the pollution rate was 3.7%.The germination rate of seeds treated with gibberellin reached89 %,and the germination potential and germination index of Actinidia argutat seeds were significantly improved,and seedlings emerged earlier.(2)In the cutting propagation,it can be concluded from the orthogonal test analysis that the semi-lignified branches of the current year are cut as cuttings,and the cuttings are placed in the moss.Rooting works best.(3)In the process of tissue culture,the best disinfection method for stem segments is alcohol disinfection for 30 s,followed by immersion in 0.1% mercury chloride for 7minutes.Plants in MS basic medium grew robustly,with tender green shoots and short germination time.With MS+2.0 mg/L 6-BA+0.1 mg/L NAA as the best induction conditions,the germination rate of axillary buds in the stem segment reached 91.56%.The conditions suitable for the proliferation of clump shoots were MS+0.2 mg/L IBA+2.0 mg/L 6-BA,and the proliferation coefficient was 4.37.In the rooting stage,1/2MS was used as the basic medium,and 0.4 mg/L IBA was added to the rooting stage.The largest,the average number of taproots was 6.48,and the average root length was3.60 cm.The tissue culture seedlings after rooting were transplanted into moss,and the survival rate was 90%.(4)In the study of pollination ecology,the stigma receptivity was the strongest at full flowering stage,and then gradually weakened.Compared with TTC staining and I2-KI method,in vitro culture method is more suitable for identifying pollen vitality.The pollen at room temperature is completely inactivated after 7 days,and the pollen at4 ℃ still has 50% vitality after 30 days of refrigeration.The fruit size and the number of seeds per fruit obtained by artificial normal pollination were also significantly higher than that of natural pollination.Chinese honeybee of the genus Apis mellifera is an effective pollinator of Actinidia argutat.