| Purple sweet potato is a special breed of Ipomoea batatas(L.)Lam.,with unique hereditary character and economic value which is well known for its purple tuberous root. Chemical analysis shows that the purple pigments are cyanidin derivatives.At present,the study about the genes expressions,signal conduction and regulation involved in anthocyanin biosynthesis was focused on the flower,fruit and seed.The roots and the tuberous of purple sweet potato can accumulate anthocyanin even in dark circumstance.Obviously,the regulation mechanisms in purple sweet potato root or tuberous root are very different from others;maybe there are new regulation mechanisms.There are so many factors involved in anthocyanin biosynthesis,the light is one of the most factors that regulate anthocyanin accumulation,so that it's very interesting to discover the pathway that light regulate anthocyanin biosynthesis in purple sweet potato root or tuberous root which grow up in darkness.And if we get the law,we can regulate anthocyanin biosynthesis in purple sweet potato or other plants with some biotechnology.In this experiment,the purple sweet potato "Yamakawamurasaki" and the white flesh sweet potato "Yubeibai" were used as test materials.Firstly,the liquid culture system of purple sweet potato was established,and then the anthocyanin accumulation and the genes expressions involved in anthocyanin biosynthesis of the two test material in different light environment were analyzed.The results are as follows:1.Establishment of the liquid culture system of purple sweet potato.In this experiment,a purple sweet potato cultivar "Yamakawamurasaki" was used as test materiel to study factors that affected the growth of cuttings during liquid culture by measuring some indexes,such as plant height growth,leaf number,leaf area,root length,root number and root fresh weight. The experiment results and one way analysis of variance(ANOVA)indicated that the cuttings from middle stem section grew much better in Hoagland nutrient liquid than the cuttings from the top or base stem section;and the 1/2 Hoagland nutrient liquid was better than the water, 1/4 Hoagland,Hoagland,1/4 MS,1/2 MS and MS to In vitro culture the cuttings from purple sweet potato. 2.Light regulate the anthocyanin biosynthesis in purple sweet potato.All illumination, up ground illumination promote the anthocyanin accumulation in "Yamakawamurasaki" and "Yubeibai" leaf,petiole and stem;underground illumination and dark treatment inhibit.All illumination,up ground illumination and underground illumination promote the anthocyanin accumulation in "Yamakawamurasaki" root,dark treatment inhibit.The up ground part is the main place to receive the light information.3.Light affect the sugar accumulation of purple sweet potato.All illumination and up ground illumination promote sugar accumulation in "Yamakawamurasaki" and "Yubeibai" leaf,petiole,stem and root,but underground illumination and dark treatment inhibit.The up ground part is the main place to receive the light information and photosynthesis.4.Some genes involved in anthocyanin biosynthesis are regulated by light in "Yamakawamurasaki" and "Yubeibai" leaf,petiole,stem and root,some not;and the place to receive the light information are different,some are up ground part,some are up ground and under ground.The genes,such as CHS in "Yamakawamurasaki" and "Yubeibai" petiole,CHI in "Yamakawamurasaki" root and "Yubeibai" leaf,petiole and root,F3'H in "Yamakawamurasaki" root and "Yubeibai" petiole and root,DFR and ANS in "Yubeibai" petiole,are not regulated by light,but the other genes are regulated by light.The genes,like CHS,CHI,F3H,F3'H in "Yamakawamurasaki" leaf,CHI,F3H,F3'H,DFR,ANS in "Yamakawamurasaki" petiole,and CHS,F3'H,DFR in "Yubeibai" leaf,and the all six genes in "Yubeibai" stem,receive the light information through both the up ground and under ground part,but DFR and ANS in "Yamakawamurasaki" leaf and all six genes in "Yamakawamurasaki" stem just through the up ground part.
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