Analysis Of The Nutrition Ingredient Of Prinsepia Utilis Royle Fruit Meal And The Immunoloregulative Effects On Chicken

The relationship between the immune and nutrition gradually being revealed,Not only for animal nutrition to maintain normal growth and development necessary,but also to maintain the immune system function and the decisive factor is immune activity is fully expressed.Therefore,the nutritional immunology research have gradually become the hot spot.With the nutritional immunology to development gradually,people to turn their attention to the nature of herbaceous plants,Because it provides a balanced,pure nutrition is the best energy to enhance immune function. Through a lot of information that Prinsepia Utilis Royle contains rich nutrients such as water,ash, crude fiber,fat,protein,17 kinds of amino acids,vitamins A,D,E,K,and a variety of microelement. In China,Prinsepia Utilis Royle is rich in resources,it provided a good condition.for research, development and use.But a long time,after Prinsepia Utilis Royle squeezed the Prinsepia Utilis Royle oil,the Prinsepia Utilis Royle Fruit Meal blank in the development and utilization of state.So, study the immunoloregulation effects of Prinsepia Utilis Royle on chicken,by the animal experiment and the nutritional components analysis of Prinsepia Utilis Royle Fruit Meal such as amino acid,crude protein,crude fat,vitamin A and microelement.The main contents are as follows:1.Analysis of the Nutrition Ingredient of Prinsepia Utilis Royle Fruit Meal Analysis of the Nutrition Ingredient of Prinsepia Utilis Royle Fruit Meal at first time in this experiment.Analysis of the amino acid,crude protein,crude fat,vitamin A and the microelement by the methods of the amino acid autoanalyser,Kjeldah method,Soxhlet,high efficiency liquid chromatography and atomic absorption spectrotype,respectively.The results showed that the Prinsepia Utilis Royle Fruit Meal was a good material with rich nutrients,the content of amino acids is 36.83%,36.83%,the content of crude protein is41.26%,the content of crude fat is 6.12%,the content of vitamin A is 35.40mg/g,the content of microelement Fe,Zn,Mg,Mn,Cu is 122.29 ug/g,54.64 ug/g,2.47 ug/g,35.01 ug/g,9.38 ug/g.The results prove that the Prinsepia Utilis Royle Fruit Meal is rich in nutrition which has immune-related components.; 2.The Immunoloregulative Effectsof Prinsepia Utilis Royle Fruit Meal on Chicken Animal experiment:Sixty 1-day-old chicken were divided into three groups randomly.Group I feeded with basal diet.GroupⅡand groupⅢwas supplemented with 10g/kg and 15g/kg Prinsepia Utilis Royle Fruit Meal in the basal diet respectively.After 14d,21d and 28d the group which effect of weighting is best was picked out to blood and immune organize(thymus,spleen,bursa)sampling.Blood be use to mensurate erythrocyte C3b receptor ratio(E-C3bRR),erythro- cyte immune compound ratio (E-ICR),Et,Ea,percent of T lymphocytes,Antibody level(IgG,IgA,IgM)and Blood Biochemical Indicator(Glutamic Oxalacetic Transaminase(GOT),Glutamic Pyruvic Transaminase(GPT),serum calcium,Alkaline Phosphatase(AKP),Seralbumin(SA)).Immune organize be used to mensurate the index of immune organ,the quantity of T lymphocyte and macrophage.And then we detected change of structure after making splices and Hematoxylin and Eosin(HE)staining.Otherwise ultrastructure of spleen was be observed under electron microscope.The results showed that(1)the effects of Prinsepia Utilis Royle Fruit Meal on the sera antibody and blood biochemical indicator in chickens as flow:Comparable with control group,The content of IgG increased significantly after taken Prinsepia Utilis Royle Fruit Meal.The content of IgA had no significantly change,but it increased comparable with control group;The content of IgM has been enhanced significantly in the 21th day.The content of IgM increased comparable with control group in other days.The content of GOT,GPT,AKP increased significantly in the 14th day,and following the experimental proceeding,it reduced to common gradually.The content of serum calcium and SA have no significant change.(2)The effects of Prinsepia Utilis Royle Fruit Meal on the immunity of RBC and lymphocyte in chickens as flow:The rate of E-C_3bRR and E-ICR have no significantly change comparable with control group,and the rate of ANAE+ stain T lymphocyte,erthrocyte active rosette and erythrocyte total rosette all increased significantly in each age(P＜0.01).(3)The effects of Prinsepia Utilis Royle Fruit Meal on the immunocompetent cell in immune organ in chickens as flow:Compared with control group,the percent of T lymphocyte in experimental group increased significantly in the early days at 14,21d(P＜0.05 or P＜0.01).The percent of macrophage in experimental group increased significantly in later period at 21,28d (P＜0.05 or P＜0.01).(4)The effects of Prinsepia Utilis Royle Fruit Meal on the histology of immune organ in chickens as flow:Compared with control group,the weight of thymuses,bursas of Fabricius and spleens of experiment group increased significantly(P＜0.05)or extremely significantly(P＜0.01).Under light microscope,at 14-old-day,the nodulus lymphaticus formed,cortex of thymus is thickening in different lever,spleen Periarterial Lymphatic Sheaths formed and the demarcation between cortex and medullary substance in bursa of test group was more clear than control group;At 21-old-day, the cell differentiation of lymphocytes is maturate than control group,and it has small appearance, and nodulus lymphaticus increased diameter,the number of Hassalls corpuscle more than control group,the thymus cortex broadened.Lymphocytes in spleen align closely;At 28-old-day,the nodulus lymphaticus in bruse and concentric corpuscles of test group was bigger than control group, Langerhans cell have been appear.The germinal center and splenic nodule in test group become prosperous.Under electron microscope,the population of nucleolus increased and nuclear chromation moves around the nuclear membrane and lymphocytes with uniform form in spleen lymphocytes of test group.The activity of lymphocytes increased as time grow.The results prove that Prinsepia Utilis Royle Fruit Meal has no harmful effects on blood serum biochemical indicator,that it have these function:the level of blood serum antibody could be raised, there was not blight on blood biochemical indicator;there was active regulating action on cellular immunity;it was useful for growth and maturation of immune organ.It is thus evident that Prinsepia Utilis Royle Fruit Meal has rich nutrients which boriler chicks needs.It has Immunomodulation effect for growth of immune organize on boriler chicks in it's early times.