Isolation And Identification Newcastle Disease Virusisolate In Yulin And Preparation Of Oil-Adjuvant Inactive Vaccine

Newcastle disease is a highly fatal and contagious avaian disease caused by Newcastledisease virus. The main measures of prevent poultry from ND is vaccinatted them with vaccine.According to the material indicastd, The non-model Newcastle disease is in popular in manypalcesOutside this unreasonable immunity vaccination, It maby was caused by the diffrentgenetype NDV with vaccine swain.Four Newcastle disease virus strains was isolated,identified from four local poultryfarms, named YL01,YL02,YL03,YL04. Result of identification shows YL03 the ELD₅₀is 10^-8.5/0.1mL; MDT is 42 hours and ICPI is 1.89. all the character of the isolate indicates strainYL03 is strong virulent one. The F gene of the isolates NDV was amplified by RT-PCR, theresult of nucleotide and deduced animo-acid sequence shows the cleavage site of the peptidewas ¹¹²R-R-Q-K-R-F¹¹⁷. All the results shows the YL02,YL03,YL04 belongs to gene Ftypeâ…¦in Avian paramyxovirus 1 of NDV.The oil-adjuvant invactive vaccine with strain YL03 was preparated. The vaccineimmunized local poultry, after immunization 14 day, the chickens was inooalated with the YL03and F48E9 virus, the incidence of protection is 100% in the immunized group, the mortality is100% in the control group. It shows that the oil-adjuvant vaccine is effective.