Fine Mapping Of Two Important Rice Genes Using Two Sets Of Single Segment Substitution Lines

Chromosome single segment substitution lines (CSSSLs) are a set of near- isogenic lines, which are developed by using backcross and marked-assisted selection (MAS). CSSSLs can eliminate genetic background interference, so they are very important experimental materials which are widely used in analyses of quantitative trait loci (QTL).By the aid of backcross and MAS, we developed two sets of CSSSLs about chromosome 9 and chromosome 11 of rice, using Lemont as recipient and Teqing as donor. Investigating some important agronomic traits of these two CSSSLs, we discovered one QTL which control tiller angle in chromosome 9 and one major gene which contribute to resistance to rice stripe disease.Tiller angle of rice is one of important target characters in shaping ideal plant type. It directly connects the grain yield and occurrence of diseases and inset pests on lower leaf sheath of rice plant. So studding the gene of control tiller angle is very important to creat ideal plant type of rice. A QTL contributing to tiller angle was localized to a 97.238Kb region by two molecular markers, 79.6-9-9 and 79.6-9-5, on chromosome 9.Rice stripe disease is a destructive disease in Jiangsu province nearly years. A major resistance gene was discovered on chromosome 11 from Teqing through investigating the resistance of the CSSSLs to the disease. This gene was localized to a 129.674Kb region by two molecular markers, 70.3-39C and 70.3-2.