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Establishment Of A Core Collection For Adzuki Bean [Vigna Angularis (Willd) Ohwi & Ohashi] Germplasm Resources And Its Genetic Diversity Assessment

Posted on:2009-08-05Degree:MasterType:Thesis
Country:ChinaCandidate:N XuFull Text:PDF
GTID:2143360245465090Subject:Crop Germplasm Resources
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Adzuki bean[Vigna angularis (Willd) Ohwi & Ohashi] is one of the main food legume in China, and play a particular role in people'lives. The number of accessions of Chinese Adzuki bean is the largest in the world, however, as a minor crop, little fundamental study and utilization about adzuki bean germplasm resources was done. In this study, an adzuki bean core collection was established, which was validated to be a representative sample of the entire collection. We assessed the core collection using morphological traits and SSR molecular markers to know the genetic diversity, the origin, spread pattern of adzuki bean in China. The main results were as follows:1. Establishing a core collection of adzuki bean can simplify management and enhance utilization of genetic resources in adzuki bean research programmes. In this paper, Unweighted Pair-Group Method Using Arithmetic Averages(UPGMA) was used to establish an adzuki bean core collection based on geographical distribution and phenotypic data.Finally,8.92% of adzuki bean accessions were selected from entire collection as a core collection of 435 accessions sharing 98.3% phenotypes with the entire colletion. The phenotypic correlations among different traits that may be under the control of co-adapted gene complexes were also preserved in the core collection. By different statistical methods such as comparison of mean using t test, variance using F test, phenotypic frequency distributions using Chi-square test for the different traits, the core collection was validated to be a representative sample of the entire collection. This core collection will play an important role in the utilization of adzuki bean genetic resources.2. Analysing the diversity of 396 accessions of adzuki bean germplasm resources based on morphological data for 12 traits: The mean of Shannon-Weaver'information index of adzuki bean in China was 1.158, Hubei, Shannxi, Anhui were the first three higher provinces, which were concluded to be the diversity centres of adzuki bean in China, respectively, were 1.653, 1.554, 1.544. All materials were divided into 4 groups. Materials in the first group were all from Northeast.The second group included materials from Northeast, North region of China and drainage area of Yellow River. Materials in the third group were mainly from drainage area of Yangtse Rive. The fourth group included materials mainly from Southwest. All 22 populations were divided into 5 groups, which was similar to the 4 groups. The clustering groups had an obvious relationship with geographic distribution of Chinese adzuki bean ecotypes.3. Assessing the genetic diversity of 375 accessions of adzuki bean germplasm resources based on SSR markers: 13 pairs of SSR markers producing polymorphism were screened, which contained 7 pairs of adzuki bean SSR markers, 4 pairs of mungbean SSR markers, 1 pair of cowpea SSR marker, and 1 pair of common bean SSR marker. The mean of Polymorphism information conten(tPIC)was 0.561, 4~19 alleles were detected with an average of 10.23 alleles per SSR primer pair. All materials were divided into 11 groups, the clustering groups had no obvious relationship with geographic origin. All 20 populations were divided into 6 groups, the clustering groups had an obvious relationship with geographic origin. The materials from Northeast had a closer genetic relationship with the materials from Middle-South region than the materials from North region of China, middle and lower reaches of Yellow River.4. SSR data showed that Hubei, Shannxi, Anhui were the first three higher PIC provinces, respectively, were 0.687, 0.640, 0.621, which was in accord with the morphological data. The gene flow between Hubei and Shannxi, Hubei and Anhui were all frequent, we concluded that Hubei, Shannxi, Anhui were the origin or diversity centres of cultivated adzuki bean in China. There were three possible spreading ways from the origin for adzuki bean in China: first, beginning from Hubei to Shannxi, crossing Neimenggu to Northeast; second, beginning from Hubei to Anhui, crossing Shandong, Henan to North region of China; third, spreading to Hunan, Sichuan, etc.. Cultivated adzuki bean from Southwest, especially Guangxi, Yunnan maybe had another origin.
Keywords/Search Tags:Adzuki bean, core collection, morphological traits, SSR molecular marker, genetic diversity
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