Application And Development Of The Differential Medium For Fusarium Oxysporum F.sp. Cubense Race4

Fusarium wilt of banana(Panama disease) is an important threat to the development of banana industry in China. A rapid detection technology of Fusarium oxysporum f.sp. cubense will contribute to early diagnosing and effective monitoring of the disease.From 2006 to 2008, the rapid detection technology of Fusarium oxysporum f.sp. cubense was studied and achieved the main results as follows:1 .Developed KM differential medium. KM medium was prepared by modifying the formulation of KOMADA and K2, It was a differential medium for detection of. Fusarium oxysporum f.sp. cubense race 4. The pathogenic fungus formed gear edge colony on KM medium which is an important diagnosis characteristics. KM differential medium is highly selectivity, effectively inhibited other microorganisms and promoted the growth of Fusarium oxysporum f.sp. cubense race 4.2. Application of the differential medium. KM medium was tested in the field of fusarium wilt of banana disease, and the isolates were detected from diseased banana tissue and soil by gear edge colony. The isolates had been proved to be Fusarium oxysporum f.sp. cubense race 4 by morphological identification, pathogenicity tests, vegetative compatibility tests, DNA-ITS and RAPD-PCR analysis. KM differential medium was proved to qualify for identifying Fusarium oxysporum f.sp. cubense race 4 from infected tissue and soil in the application tests, the technique was a rapid, reliable, convenient and practical detection method.The detection technology of KM differential medium will integrate with the pathogenicity tests, vegetative compatibility tests, DNA-ITS or RAPD-PCR analysis to establish a detection system of Fusarium oxysporum f.sp. cubense race 4.