Study On The Extaction Of The Total Flavonoids In Bee-Pollen

Here use bee pollen and rap pollen as material,research in bee pollen flavone category substances extracting,and discuss the influence of elements such as solvent density,extracting temperature and time,meterial and liquid rate,stir speed,etc,on extracting rate,find the best conditions of bee pollen flavone category extracting through orthogonal experiment and DPS analysis:temperature-35℃,ethanol density-65%, extracting time-32h,material and liquid-6:1,stir speed-rev/min.Under this,tee pollen extracting rate is 55.52%,as rape 48.86%.Stbstances of flavone category of bee pollen are kinds of secondory metabolities in plants growth,they are important hysiological active ubstances of pollen.Through comparing with other examining ways,we make color comparing as the way of maesureing the pollen total flavone in bee pollen.And the examing wavelength is 420nm,if use rutin as standard,under certain density range,the density and absorbency appear a good linear relation,the reletive coefficient is 0.99991,and stability,precision,repeatability are satisfitied.Under this condition,the average total flavone contentin ethonal extracting substances of bee pollen is 30.28%,as in rape pollen is 33.62%.