Study On Source And Elimination In Royal Jelly Drug Residues In The Colony

Antibiotic residues in bee products is one of the key problems, in particular when the European Union implement the green barriers on China. In bee products How to control antibiotic residues is a key problem to solve. Our aim is to exploreTetracycline and OxyTetracycline metabolism invitro, after feeding Tetracycline and OxyTetracycline to bee colony ,and every 10 days , royal jelly, worker hypopharyngeal gland and hemolymph were picked up, then analyzed by HPLC individually within 60 days.The results show that the Tetracycline and OxyTetracycline residues in royal jelly are gradually decreased with time. The Tetracycline residues in royal jelly are basically true to European Union standards 40 days later. The OxyTetracycline residues are completely compliance with European Union standards 50 days later. It was discovered that the work hypopharyngeal gland also have antibiotic residues, and we certain that part of residues are from hypopharyngeal gland. In the hemolymph of worker body are not found the antibiotic residues.1 Antibiotic residues in Royal JellyEvery 10 days we selected the samples after feeding antibiotics, and use HPLC to analyze we found that Tetracycline residue is 0.0532mg/kg after 40 days, it already achieve the standards of EU and other countries. The antibiotic residue is 0.0391mg/kg after 50 days, it is fully accord with other countries 0.05mg/kg standard. Feeding the OxyTetracycline to the honeybee colony, we found that the antibiotic residues is 0.0597mg/kg, in line with basic 0.05mg/kg standard. After 60 days we detected that the residues reduce to 0.0482mg/kg, it is full compliance with standards.2 Antibiotic residues in hypopharyngeal glandThe hypopharyngeal gland sampled from the worker after feeding antibiotics for 10 days ,deterrmined by HPLC, we found that hypopharyngeal gland also has antibiotic residues, the residue of Tetracycline achieve to 0.0678mg/kg, and Oxytetracycline residue reached to 0.0832mg/kg. We conclude that part of residues are from worker hypopharyngeal gland.3 Antibiotic residues in hemolymphFeeding antibiotics to bees , we sampled the hemolymph, conducted by HPLC detection, the residues was not found in the hemolymph of workers body.Clearly, antibiotic residues in royal jelly primarily form worker hypopharyngeal gland and combs. We found that after 50 days royal jelly is in line with pesticide residues standards.