Transformation Of SaNHX Gene Into Kenaf And Primary Identification Of Salt-tolerance

Soil salinization is a cosmopolitism problem. To cultivate and grow salt tolerant crop increasingly become an urgent and substantial research topic. This study selected the SaNHX gene which was cloned from Spartina anglica by Crop Genetics and Breeding Institute of Fujian Agriculture and Forestry University as a genetic transformation of the target gene, used pollen tube pathway, injected salt-tolerant gene SaNHX into new kenaf variety of fuhong992, and it has already expressed. The major results were as follow:(1) Using Ovary Injection, adopted 2 dosage and 3 concentration of plasmid transducted Fuhong 992, deal with 1933 flowers through microinjection treatment. The results indicated: in transgenic T₀ generation, there was no significant difference between the setting seed rate and the emergence rate of the 2 different dose. The setting seed rate and the emergence rate of T₀ generation of 3 concentration of plasmid have significant difference. The best setting seed was Z-100-8 to reach 65.5%; the best seedling emergence rate of T₀ generation is Z-300-8 to reach 81.1%.(2) Of transduction To generation, extracted DNA of 282 strains test materials to carry out salt tolerance PCR molecular detection, detected that 9 positive plants had objective gene characteristic bands, The transformation rate was 3.19% through this method. Under 0.71% concentration salt stress treatment, seed germination rate, and growth potential of these 9 positive plants were obviously better than the control, the increment of its seedling height , seedling weight and seedling root length were increased 2.04cm, 0.23g and 3.7cm. The results showed that pollen tube pathway was an effective way in kenaf transgene.(3) Variance analysis of different concentration of three different periods showed that seedling survival rate of Salt-toerance transgenic T₁ generation were obviously better than common control and differences all reached very significant level; the best was 176 strain, its seedling survival rate were 100%, 85% and 60% respectively under three concentrations: 0.3%,0.5% and 0.71%. Under Salt Stress (0.71%) , the height, the weight and the root length of seedling growth of 10 days were obviously better than the control.Of all the 9 transgenic strains, the best was No. 176 strain, the increment of its height, weight and root length were 5.47cm,0.72g and 4.54cm higher than those of control respectively.