Construction Of Three Genetic E-maps And The Preliminary Research Of The Fringerprinting Database In Rice

In order to improve the gene mapping efficiency in Minghui 86 background.we construceted three genetic linkage Map using the program Mapdraw between minghui86 and the indica rice 9311,the japanica rice 02428 and zhong hua 15 which have the conpensively compatibility,,(I accept the work of the linkage map of six rice chromosomes.The other six were constructed by another student).At the first we selected 716 published SSR primers to test the polymorphism between ming hui 86 and 93-11,ming hui 86 and 02428,ming hui 86 and zhonghua 15.In order to shorten the distance between the various markings we developed 516 pairs of primers using the rice genome sequence in this study with the help of software SSRIT and primer 5.0.Of all the 1232 markers,281 primers showed polymorphism in the size of PCR products between Minghui 86 and 93-11,and 473 primers between Minghui 86 and 02428,and 470 primers between Minghui 86 and Zhonghua 15.Polymorphism frequencies were 22.81%,38.39%and 38.15%. According to the electronic genetic distance of these markers in the chromosomes,we constructed three SSR electronic genetic map using the program Mapdraw,the totle chromosome length covering of these markers were:805.4cM,815.4cM, 813.3cM,and an average distance between adjacent markers was 5.34 cM,3.22cM and 3.23cM,respectively.The three high-density genetic maps might facilitate the work of high-throughput gene mapping for the rice Minghui 86's mutant and also be helpful for map-based cloning of genes in the other three cultivars.In the process of the linkage map construction,According to the polymorphism in the size of the pcr products in the four varieties(ming hui 86,9311,02428,zhonghua 15),we select some markers with high polymorphisms.And then,we test the polymorphism by amplifying the cultivars which including 5 cultivars of restoring line,4 cultivars of maintaine lines and 3 cultivars of japonica.According to the polymorphism and amplified stability of the PCR analysis,We identified 72 markers,they distributed in the 12 chromosomes on everage,and each arm of one chromosome have 3 markers.All the work laid the groundwork for species identification and the construction of these cultivars' fingerprinting.