| In order to improve the gene mapping efficiency in Minghui 86 background , A total of 1232 pairs of SSR primers, of which 716 pairs were selected from the published SSR primers and 516 pairs newly developed using rice genome sequence in this study, were used to detect polymorphism between Minghui 86 and the other three cultivars. Based on these polymorphic markers and their location in chromosomes (partly markers such as direct quote McCouch reported the genetic distance, another part of a newly developed genetic markers from the physical distance is based on caculation) three base on Rice Cultivar "Minghui 86" genetic E-maps were constructed using the program Mapdraw.evenmarker(Unlike traditional analysis of genotype and the combination to construct the genetic map, so we named genetic e-map) Minghui86/93-11,Minghui 86/02428, Minghui 86/Zhonghual5 E-map comprise 281,473 and 470 microsatellite maker loci, with an average distance of only 5.34 cM, 3.22cM和3.23cM between adiacent markers re respectively. The partial three genetic maps of the 6 chromosomes from 4～9 containl33,200 and 214 microsatellite maker loci,with an average distance of 5.22 cM,3.53 cM,3.35. The three high-density genetic maps might facilitate high-throughput gene mapping in Minghui 86 background and also be helpful for map-based cloning of genes in the other three cultivars.At the same time , A total of 1232 pairs of SSR primers were used to analyse polymorphism in 12 Relationship representative rice varieties,and 72 pairs corn markers were selected on the 12 chromosomes(each chromosome 6).36 pairs of corn markers have been selected in this study, the 36 pairs of corn markers could amplify 122 alleles totally , the number of alleles per locus ranged from 2 to 6,with an average of 3.39 alleles per locus. The three sets of cron primers amplified bands of clear, good stability, high polymorphism will lay the the foundation for establishment of a automation varieties identification system. |
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