Study On In Vitro Culture And Plantlet Regeneration With Stem And Leaf Explants From Actinidia Arguta Planeh.

The surface was hairless. It's can be directly eating and processed into canned fruit, preserved fruits, high value. Arguta was the best breeding materials. Efficient plant regeneration system is founded to success for transformation with arguta. The shoots isolated from kiwifruit were used as explants in this study. By means of the orthogonal design, analysis of variance and multiple comparisons, the factors, including basic media, plant growth regulators, seasons, were analyzed and the ideal regeneration system of arguta was established. The results were as follows:(1)The Organogenesis trip was so good, But the Organogenesis trip was cost more time. And the Organ trip was more Stability.(2)The stem was the Organogenesis trip and the most fitting season to gather the stem was spring. To compare the differentiation of different callus from stems, petioles and leaves, the differentiation rate of callus from stems was 45.41%. However, it was 15.5% from leaves. To compare the browning rates of different callus from stems, petioles and leaves, the differentiation rate of callus from stems was 30.8%. Leaves were the highest. On budding rate, stem, leaf stalk, leaf piece difference are notable, stem reaches 85.5%, be taller than most. The petioles was lower than the stem, the leaf piece was lowest with 6.37%. Budding force aspect, petioles, leaf piece difference are not notable, it has notable difference with the stem.(3)MS medium was more suit as basic medium than the others by stem callus induction. On the stage of callus induction, the MS medium supplemented with 3.0 mg/L BA and 2.0 mg/L IBA was the best one. On the stage of buds differentiation, the best medium was the MS medium containing 3.0 mg/L BA plus 0.2 mg/L IBA with bud regeneration rate of 86.37%. In the root regeneration trial, 1/2MS medium with 0.2 mg/L NAA was more beneficial than the others of 85.89%. To get more cuvette plants, the best multiplication medium was the MS medium containing 2.0 mg/L BA plus 0.4 mg/L NAA with the multiplication rate of 5.33.(4)The leaves of cuvette plant to regenerated buds, the optimal initiation medium was the MS medium supplemented 2.0 mg/L BA and 0.3 mg/L NAA. And the bud regeneration rate was 87.06%.(5) The regenerated plants with roots were planted in the medium containing 25% vermiculite, 25% perlite, 20% sand and 30% soil with the survivor rate of 90% after a month.