Study On Rapid Propagation Technique In Vitro Of Actinidia Arguta

This study takes Actinidia arguta as test materials. To build effectively rapid propagation clone in vitro of Actinidia arguta,which include primary culture, subculture proliferation, rooting culture,domestication and transplant culture. Studied the effect of explant of sterilization time,the kinds and consistency of growth regulator, subculture cycle and times of subculture and transplanting media. In order to provide technical support for raising seedlings in industrial scale. The experimental results showed that:1. Using branches germination of axillary bud, terminal bud and stem section as explant. With 0.05% mercuric chloride explant for different time sterilization, the best sterilization time of axillary bud and terminal bud was 4min,the best sterilization time of stem section was 6min.2. In the process of initiation culture.The best germination with axillary bud for stem section was MS+2.0mg/L 6-BA +0.1mg/L IBA, the germination rate was 92.37%, and the germination number is 3.43 thick green and bud growing strong.The optimum initiation medium of terminal bud was MS+1.5mg/L6-BA+ 0.25mg/LIBA,initiation rate was 96.19%.3. There was multiplication culture by shoot cluster proliferation and stem section cottage proliferation.The optimum proliferation medium of shoot cluster was MS+2.0mg/L6-BA+0.2 mg/LIBA.The proliferation coefficient was 4.69, and shoot cluster grow robust, leaf luster green.The optimum proliferation medium of stem section cottage2.0mg/L6-BA+0.3 mg/LIBA stem growth status strong, stem length 6.0-7.0cm, the proliferation coefficient can achieve 7.28. In the process of trans generational multiplication culture.The best trans generational cycle was 40 d.The growth modulus was 4.72. Transfer times should not be more than 6 times. Planting density was every bottle inoculated 9individual, proliferation coefficient was 4.67. Tissue culture seedlings grow strongly and uniform, stem tenacity.4. The best medium for seedling cultivation of MS as the basicculture medium and add growth regulator2.0mg/L6-BA+0.2mg/LIBAand+30g/Lsucrose. Shoot cluster grow strongly and uniform, leaves large and flat. It was beneficial for the next tissue culture of rooting culture.5. The best medium of rooting culture was 1/2MS+0.4mg/LIBA+30g/L sucrose +2.0g/L activated carbon. Add 2.0 g/L of activated carbon to make the tissue culture seedlingswithout callus and plants strong, make the root system tough and radiated, the rooting rate reached 100%, average root number at best was 8.09.6. The optimum transplant media was garden turfy soil:soil: perlite=2:2:1(V:V:V). With transparent film mulching on transplanting seedlings to keep the air humidity. The survival rate of transplant was96.36%, transplanting seedlings grow strong, the leaf rich luster and stem robust.