Effects Of Rare Earth-chitosan On Lipid Metabolism And Growing Function In Sichuan Bone-black Chicken

The Experiments were carried out to study the effect of Rare earth-chitosan on growth performance,lipometabolism,serum biochemical parameters,material metabolic rate of nutrients and Growth-promoting effect in Sichuan Bone-black chickens.A total of 240 one day old Sichuan bone-black chickens were sampled and randomly allotted to 5 dietary treatments(12 birds per pen with 4 pens per treatment); the experiment period were 56days,for a two-phase trial(0-4week,5-8week).The treatment group 1(control group) was treated only with basal diet;treatment 2 group adding the chlortetracycline(CTC)(50 mg/kg),in contrast,the treatment groups 3,4 and 5 were designed by adding the Rare earth-chitosan into the basal diet with 0.10%, 0.15%and 0.20%dosage,respectively.After determining the fitting addition dose, an another total of 192 one day old Sichuan bone-black chickens were further sampled and randomly allotted to four dietary treatments as described above;the experiment period were 56 days.The treatment groupâ… (control group) was treated only with basal diet.On the contrary,the treatment groupsâ…¡,â…¢andâ…£were conducted by adding 0.10%Rare earth-chitosan,0.08%Chitosan,and 0.02%Rare earth into the basal diet,respectively.The result indicated that: 1.The growth performance of Sichuan bone-black chicken was improved by Rare earth-chitosan and chlortetracycline respectively.In contrast to the CTC group, Rare-earth Chitosan treatment group additives 0.10%increased 3.09%the average daily gain(ADG) in the 0-4 weeks;Rare earth-chitosan group additives 0.10% increased 4.88%the ADG in the5-8 weeks;and it increased 3.55%the ADG in the 0-8 weeks.In the 0-4 weeks,average daily feed intake(ADFI) of the Rare-earth chitosan were no significantly difference among other treatments.5-8 weeks and 0-8 weeks had no significantly difference compared with the CTC group.In the 0-4 weeks,5-8weeks F/G was reduced by 3.08%,3.52%(P<0.05) compared with the CTC group.The further experiment showed that,in contrast to the Chitosan group and Rare-earth group,Rare earth-chitosan treatment group additives 0.10%increased the ADG and ADFI in the 0-4 weeks,5-8weeks and 0-8weeks;in the 0-4 weeks, 5-8weeks F/G was much lower than other treatment groups.The results showed that the better increased ADG and improved feed conversion were Rare-earth chitosan additives 0.10%and the CTC group.2.The Rare earth-chitosan additives were increased content of TP and ALB,reduced content of BUN and AKP,and there were no significant differences on the content of TP,ALB,BUN,and ALT between treatments.The further experiment showed that,Rare earth-chitosan additives were increased the content of TP and ALB much higher than Rare-earth group and chitosan group.3.In contrast to the CTC group,the metabolic rate of CP of Rare earth-chitosan additives 0.10%increased 3.22%;the metabolic rate of ME increased 7.47%(P < 0.05);DM increased 7.66%(P<0.05),the metabolic rate of fattiness reduced 1.21%.The further experiment showed that,In contrast to the Rare earth and chitosan group,the metabolic rate of CP of Rare earth-chitosan additives 0.10% increased 8.83%(P < 0.05),3.17%(P < 0.05) respectively;DM increased 2.06%,1.83%respectively;the metabolic rate of fattiness reduced 0.33%,3.27% respectively.4.Rare earth-chitosan can increase the activity of ATPase,In contrast to the Rare earth-chitosan additives 0.15%and 0.20%dosage;Rare earth-chitosan additives 0.10%had the better effect.In contrast to the control group,Rare earth-chitosan additives 0.10%increased 18.64%(P < 0.01),30.15%(P < 0.01),28.39%(P < 0.01) the activity of NA⁺-K⁺-ATPase of heart,liver,kidney respectively,increased 25.93%(P < 0.01),28.03%(P < 0.01),33.33%(P < 0.01) the activity of Ca²⁺-Mg²⁺AYPase of heart,liver,kidney respectively.5.Rare earth-chitosan reduced the abdomen fat percent,liver fat percent,and the serum components ofTC,TG and VLDL-C significantly.These results indicate that the growth performance and lipometabolism,of Sichuan bone-black chickens,and Rare earth-chitosan additives 0.10%had better effect on the growth performance and lipometabolism,also Rare earth-chitosan additives 0.10%better than Rare earth additives and chitosan additives respectively.