| 41 rhizosphere soil samples were collected from the medicinal plantations of Zhongjiang(main Salvia miltiorrhiza Bunge),Dujiangyan(main Rhizoma Chuanxiong), Er'lang Mountain(main Radix cyathulae) and Sichuan Agricultural University.Soil fungi were determined with the dilution plate technique and identified based on morphologic characters.The study was undertaken to evaluate the quantities,communities and diversity of rhizosphere soil fungi of medicinal plants.In addition,the biocontrol potential of soil fungi to pathogen was also determined.There was great difference in different soil samples of 4 regions.The quantities of rhizosphere fungi were as follows:the Dujiangyan medicinal plant base(2.36×104cfu·g-1 cfu·g-1dry soil)＞the Sichuan Agricultural University medicinal plant base (2.21×104cfu·g-1 dry soil)＞the Zhongjiang medicinal plant base(1.82×104 cfu·g-1 dry soil)＞the Er'lang Mountain medicinal plant base(1.29×104cfu·g-1dry soil).There was great difference in different planting measures in quantities of rhizosphere fungi in identical region.In the Zhongjiang medicinal plant base,fungi in the mixed planting condition was extremely remarkable higher than in other ways,and there is remarkable difference between the continuous cropping and the crop rotation.Both the continuous cropping and the crop rotation is remarkable higher than the monoculture.In the Dujiangyan medicinal plant base,the mixed planting is extremely remarkable higher than he monoculture,and it is remarkable higher than the continuous cropping.Both the continuous cropping and the crop rotation is remarkable higher than the monoculture.In the Er'lang Mountain medicinal plant base,the mixed planting is extremely remarkable higher than the continuous cropping and the monoculture.The crop rotation is extremely remarkable higher than the continuous cropping.In Sichuan Agriculture University medicinal plant base,the mixed planting is extremely remarkable higher than the monoculture.Under the identical region,the quantities of rhizosphere fungi of Salvia miltiorrhiza Bunge is extremely remarkable higher than Paeonia lactiflora Pall and Bupleurum chinense DC.,and it's remarkable higher than Angelica silvestris;In Sichuan agricultural chemicals plant base,the rhizosphere fungi quantity of Radix aconiti lateralis prepapata is extremely remarkable higher than Salvia miltiorrhiza Bunge,Radix cyathulae,Ophiopogon japonicus and Angelica silvestris,and it's obviously remarkable higher than Angelica and Ligusticum,but it wasn't remarkable to Houttuynia cordata thunb.A total of 597 fungi isolates were obtained in 4 regions.Among these isolates,26 fungal genera were identified.21,17,23,21 fungal genera were found in Zhongjiang,Dujiangyan, Er'lang Mountain and Sichuan Agricultural University,respectively.Among these genera, Penicillium spp.,Trichoderma spp.,Aspergillus spp.,Cladosporium spp.,Verticillum sp.and Mucor spp.were isolated more frequently.Fungi diversity had a great difference in different regions.Shannon-wiener's diversity (H'),Pielou's evenness(E) and dominance index(D) of Zhongjiang were 61.8702±0.1011, 0.9365±0.0169 and 0.1883±0.0512,respectively.Those of Dujianyan were 61.7793±0.3977,0.9433±0.0217 and 0.2102±0.0593,respectively.Those of Er'lang Mountain were 1.7797±0.4110,0.9223±0.0719 and 0.1990±0.1052,respectively.Those of dujianyan were 1.5972±0.0810,0.9130±0.0391,0.1990±0.0173,respectively.Fungi diversity had a great difference in plant different rhizosphere in the identical region.Abundance(S),Shannon-wiener's diversity(H') and Pielou's evenness(E) in Salvia miltiorrhiza Bunge were the best,and the next is in Radix cyathulae and Rhizoma Chuanxiong,and Baizhi's diversity was the lowest.In the same region,abundance(S) and Pielou's evenness(E) of fungi under crop rotation and mixed planting's were higher than under the monoculture and the continuous cropping.Groups of fungi which were determined from the rhizosphere soil sample under crop rotation and mixed planting were rich and their distribution also even.33 isolates of Trichoderma spp.collected from the soil were used to screen the antagonist against Rhizoctonia solani.The results showed that Strain T17(Trichoderma aureoviride) had the ability to suppress R.solani,the inhibition percent of metabolites was 70.97%. |
