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Studies On New Technology Of Rapid Propagation Of Dendrobium Candidum Wall.ex Lindl In Vitro And The System Of Its Standardized Production

Posted on:2009-07-08Degree:MasterType:Thesis
Country:ChinaCandidate:G F LiFull Text:PDF
GTID:2143360245950262Subject:Integrative basis
Abstract/Summary:PDF Full Text Request
Dendrobium candidum Wall.ex Lindl, one of the Chinese traditional medicinal materials, belongs to Orchidaeae with Dendrobium as its stem. Its main ingredient is polysaccharide, alkaloid, amino-acid and etc. It is provided with efficacy of nourishing yin and clearing heat, promoting the secretion of saliva and quenching thirst, using for treating yin essence injuring and fluid deficiency, vexed thirst, lacking of appetite and vomiturition, low-grade fever, blurred vision, etc. Modern medicine study indicates that Dendrobium candidum Wall.ex Lindl has a favorable curative effect in treating diabetes, immunity control and anti-tumour.Dendrobium candidum Wall.ex Lindl, which is valuable in medication and health protection, lives in such severe environment that its wild resource is quite few to meet the market demand. Consequently, most of the Dendrobium candidum Wall.ex Lindl sold in the market now are cultivated ones. Even though there have been many Dendrobium candidum Wall.ex Lindl germchit produced by tissue culture, there is quite few successful cultivation.Recent years, tissue culture and inside and outside planting of Dendrobium candidum Wall.ex Lindl has been springing up in Guangdong area with ideal success. There are now three manufacturers devoting into planting producing. However, there are still some insufficiencies. Firstly, the manufacturers now are lack of regulations for standardized manufacturing, quality control and collection. Secondly, in the process of Dendrobium candidum Wall.ex Lindl cultivation and collection, there are many factors that will influence the herb quality such as growing environment, technique of culture of seedling, planting technique measure, breed filtering, collecting and processing, appearance quality, edible safety, packing, storage, delivery and so on.This paper will take the pilot study on the establishment of Dendrobium candidum Wall.ex Lindl SOP and GAP, analyze interrelated pharmacodynamic ingredients, and provide a producing quality control reference for Dendrobium candidum Wall.ex Lindl by research on its growing process and quality. And it will be one of the basics for producing quality criterion. Recently in our country, there are several manufacturers which are utilizing and promoting Dendrobium candidum Wall.ex Lindl artificial cultivation technique, and carrying through standardization manufacturing, but there are not any reports on feasible SOP, GAP and quality control standards. In Guangdong, Dendrobium candidum Wall.ex Lin manufacturing is so prosperous that it needs a feasible criterion that standardizes pharmocodynamic safty as quality control regulation.This paper will take the Chinese medicine tissue culturing, standardized manufacturing regulations and herb manufacturing administration regulations as reference, manager to filter the best culture medium for Dendrobium candidum Wall.ex Lindl tissue culturing, the best culture media and seed resources, and establish Dendrobium candidum Wall.ex Lindl manufacturing management regulations and standardized manufacturing technique regulations to provide a reference for filtering good quality Dendrobium candidum Wall.ex Lindl.This paper aims to search the Dendrobium candidum Wall.ex Lindl manufacturing administration regulations and standardized manufacturing technique regulations with rationalization, feasibility, regulations and standardization, and devotes to establish criterion and guidance in practical operation. It is hopeful to found an impersonality industry standard for manufacturers and supervision department.If this research can be combined with researches on pharmacological action of Dendrobium candidum Wall.ex Lindl, and provide measurements for manufacturers to cultivate, plant, and administrate so as to control its production and medication, it will bring important signification to Dendrobium candidum Wall.ex Lindl industrialization development, medicine safety, and quality guarantee.
Keywords/Search Tags:Dendrobium candidum Wall.ex Lindl, Rapid Propagation of Tissue Cultivation, GAP, SOP
PDF Full Text Request
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