Effect Of Soil Drought Stress On Drought Resistance In Three Tissue Cultured Seedling Of Anoectochilus Roxburghii (Wall.) Lind

Anoectochilus roxburghii(Wall.)lind is a rare medicinal herbs and have high enjoy value, usually it existences in place of high altitude ,loving shady and cool climate, and have a strict demand on environment, besides wilding are very exiguous,so it is very important to development cultivation.Drought environment may easily lead to unusual growth even death of A.roxburghii.So this paper taked three different sources of A.roxburghii. tissue cultured seedlings as experimental materials,setting up soil drought stress of five gradients (9,13,17,21,25 days) and 0 day for comparison, to study three kinds of A. roxburghii. tissue cultured seedlings physiological response of drought stress, then measure physiological and biochemical indexes and analyze them,using principal component analysis and subordinate function values analysis to estimate drought resistance of A.roxburghii. tissue cultured seedlings,and provide theory basis and scientific direction for numerous cultivation of A.roxburghii.Research results showd that:( 1 ) Anoectochilus roxburghii(Wall.)lind occurd an array of physiological response under drought stress, change tendency of 12 indexes is: RWC,content of Chl and Car always reduced; PMP,content of MDA and soluble sugar always increased;activity of POD,SOD,CAT,APX and content of proline and soluble protein exhibited up-down trends;APX activity exhibited down-up trends.correlation matrix of every indexes showd that every indexe had more or less correlation,and reflectd repeatd information.So this paper used principal component analysis to reduced 12 indexes for 2 components,and decide component weight,and finally proceed comprehensive analysis.(2)Through the principal component analysis and subordinate function values analysis,the results of evaluation on drought resistance of A.roxburghii shown that the ability of drought resistance of three tissue cultured seedlings of A.roxburghii. on different drought stress: S > R > OS,The result can provide direction for cultivating of A.roxburghii.