Tissue Culture And Drought Resistance Of Tissue Cultured Seedlings On Anoectochilus Roxburghii(Wall.) Lindl

Tissue culture technique and the mechanism of physiology and biochemistry under water stress on Anoectochilus roxburghii(wall.) Lindl were studied. The results were as follows:1. The best medium for stems culture was MS+BA 3.0mg/L+NAA 0.3mg/L+ sucrose 30g/L, and their multiplication needed MS+BA 3.0mg/L+KT 0.5mg/L + NAA 0.2mg/L+sucrose 30g/L. While the optimal rhizogenic culture medium is 1/2 MS + NAA 1.5mg/L+ sucrose 20g/L.2. The best medium to induce alars by rhizome was MS+ BA 1.5mg/L+KT0.5 mg/L +NAA 1.5mg/L+ sucrose 30g/L, whose multiplication required the condition of MS+BA3.0mg/L+KT0.5mg/L+NAA0.2mg/L+sucrose30g/L. Beside, the fine rhizogenic condition was 1/2MS+NAA1.5mg/L+ sucrose 20g/L.3. Under the water stress, the content of RWC, chlorophyll and dissoluble amylose of tissue cultured seedlings cultivated by overground stem and rhizome presented descending trend, MDA,Pro,POD and CAT went up first, then went down, dissoluble protein and SOD took on the change of"M"type, and APX showed ordinal change (fall, rise and fall), while the permeance property of cell membrane ascended continually. Moreover, the content of dissoluble sugar in leaves was drop, which was contradictive with the results reported before under drought stress. It meaned that fall of dissoluble sugar maybe by reason of lower anti-drought ability of Anoectochilus roxburghii(wall.) Lindl, which should be studied further.4. Using PAGE technique to analyze isozyme of Anoectochilus roxburghii(wall.) Lindl. The results showed that spectrums of POD and APX both had some changes during the process of stress, suggesting that POD and APX were possibly regulated in gene level under water stress.But the bands of CAT is comparatively stable, this is not new band .5. The drought resistance of tissue cultured seedlings of overground stem and rhizome on Anoectochilus roxburghii(wall.) Lindl were determined synthetically。On the basis of the data, the drought resistance of tissue cultured seedlings induced by overground stem was stronger than that of rhizome, which supported the result"tissue cultured seedlings of overground stem adapted to outdoor transplant better".