Preliminary Approach To The In Vitro Propagation Of Limonium Bicolor

Plant cells can be grown in isolation from intact plants in tissue culture systems. The cells have the characteristics of callus cells, rather than other plant cell types. The theory "plant cell totipotency" indicates that plant regeneration from single cell culture can be accomplished through the redifferentiation of the callus.Limonium bicolor (Plumbaginaceae) belongs to biennial forb growing in the typical steppe. It is improvement and development of Limonium bicolor tissue culture system that aseptic seedlings are used as materials. The technology is so significant for conservation of genetic diversity and living environment. It can save a great deal of manpower and material resources, improve the efficiency and quality of agricultural development. This study investigated the regeneration ability of callus from cotyledon and embryonic axis under different hormone conditions. Experimental results were as follows:1. On MS medium containing 6-BA(0.3-0.7mg/L)and NAA(0.1-0.5 mg/L), callus and adventitious bud were induced from cotyledon. The best hormone combinations were 0.3 mg/L 6-BA +0.5 mg/L NAA, 0.5 mg/L 6-BA+0.1 mg/L NAA and 0.7 mg/L 6-BA+0.1 mg/L NAA;2. On MS medium containing 6-BA(0.3-0.7mg/L)and NAA(0.1-0.5 mg/L), callus and adventitious bud were induced from embryonic axis. The best combination was 0.3 mg/L 6-BA+0.5 mg/L NAA. The longer induction time, the better differentiation of adventitious bud;3. Cotyledon was much better than adventitious bud as material of inducing callus and adventitious bud;4. MS0 medium MS medium with 0.2 mg/L NAA and 1/2 MS0 medium could induce rooting of adventitious bud, the best was MS0 medium. During this course, the requirement for NAA was at low level.