Effect Of Dietary Cu And Mn Supplement On Growth Performance, Physiological Function And The Trace Element Concentration Of The Different Organs In Carassius Auratus Gibelio

This present research was to evaluated the effect of different levels of dietary Cu and Mn to the growth performance(FCR,SGR),physiological enginery(SOD,LSZ, GPT,GOT,Hb),organic microelement on gibel carp (Carassius auratus gibelio).And this research was also to investigate the condign supplement quantity on the diet for gibel carp.In experiment 1,during the 8 weeks,the effect of different levels of Cu(2,4,6,8,10 mg/kg)in dietary on the the growth performance,physiological enginery(SOD,LSZ, GPT,GOT,Hb),microelemen(Cu,Fe,Mn,Zn)in 14 kinds of organs was investigated,and the relativity between the dietary's and the organic's Cu,Fe,Mn,Zn was else tested. The result suggested that the fish in group 6mg/kg (dietary Cu was 23.05mg/kg) perform the best SGR(1.76±0.05 %/d);As the growth of the supplement of Cu,FCR perform the increased current first and then decreased current,which performed the the best(2.00±0.08) in group 6mg/kg (dietary Cu was 23.05mg/kg).There is no difference in SOD,GOT and GPT among the groups;When the supplement of Cu exceeded 4mg/kg,the level of Hb decresed as the increase of the supplement of Cu;And the supplement of Cu was 8mg/kg,the difference in Hb was significant among groups;Liver and pancrease were the main apparatues for reserving Cu,while intestine was the main apparatues for zincZn. the level of Cu increased as the increase of the supplement of Cu in the whole body,muscle and hepatopancreas. And the supplement of Cu was 8mg/kg,the level of Fe,Mn,Zn decreased significant in the whole body, hepatopancreas and vertebra.This research suggested the condign supplement of Cu was 7.33mg/kg(dietary Cu was 24.18mg/kg).This result was based on SGR,FCR, regression of Cu in the hepatopancreas and vertebra, physiological enginery,the content of Fe,Mn,Zn in the whole body, hepatopancreas and vertebra.In experiment 2,during the 8 weeks,the effect of different levels of Mn(20,40,60, 80,100mg/kg)in dietary on the the growth performance,physiological enginery (SOD, LSZ,GPT,GOT,Hb),microelemen(Cu,Fe,Mn,Zn) in 14 kinds of apparatus was investigated ,and the relativity between the dietary's and the organic's Cu,Fe,Mn,Zn was else tested.The result suggested that the fish in group (dietary Mn was 80mg/kg) perform the best SGR(1.78±0.04%/d);As the growth of the supplement of Mn,,FCR perform the increased current first and then decreased current,which performed the the best in group 80mg/kg (dietary Mn was 139.88mg/kg).There is no difference in SOD,LSZ and Hb among the groups;When the supplement of Mn 20mg/kg, the level of serum GPT (9.88±4.11 U/10ml) was significantly higher than groups 40mg/kg, 80mg/kg,100mg/kg(p<0.05);And the supplement of Mn was 20mg/kg,the difference in Hepatopancreas GPT (216.71±45.54U/10ml) was significant among groups (p<0.05);When the supplement of Mn 40mg/kg,the level of serum GOT (112.46±13.52 U/10ml) was significantly higher than groups 20mg/kg, 100mg/kg(p<0.05); And the supplement of Mn was 60mg/kg,the level of Hepatopancreas GOT (137.37±29.10 U/10ml) was significantly higher than groups 40mg/kg,80mg/kg(p<0.05). Vertebra,Branchial bone,Crania, Kidney and Heart were the main apparatues for reserving Mn. The level of Mn increased as the increase of the supplement of Mn in the fim,spleen,vertebra, squama,whole body,intestines. The level of Cu decreased as the increase of the supplement of Mn in the whole body,brain, hepatopancreas. The level of Fe increased as the increase of the supplement of Mn in the intestines. As the increase of the supplement of Mn,the level of Zn was stabilization. This research suggested the condign supplement of Mn was 80mg/kg (dietary Mn was 139.88mg/kg). This result was based on SGR,FCR,regression of Mn in the vertebra,growth performance, physiological enginery,the content of Fe,Cu,Zn in the whole body, hepatopancreas and vertebra.