Studied On Relationship Of Velvetleaf (Abutilon Theophrasti Medicus) Germplasm By Morphology And Molecular Markers

Velvetleaf(Abutilon theophrasti Medicus), annual herb of Malvaceae Abution, is an important bast fiber plant. Studies on relationship of Velvetleaf germplasm are important for varity discrimination, merit gene discovered and widening hereditary basis. Relationship in 48 accession of Vevetleaf germplasm was assessed using morphology, RAPD and ISSR markers.The results are followed:(1) Morphologic clustering analysis showed that the materials can be divied into two groups.The first group included 38 cultivated species. The second group included 10 wild species. This result is similar to the result of common classification.(2)17 primers selected from 100 primers amplified 191 RAPD fragments and 167 bands of them showed plolymorphism, which occupied 87%. The dendrogram was constructed by Unweight Pair Group Method with Arithmetic Mean based on their genetic diversity calculated from RAPD data. It was concluded than the 48 breeds could be classified into three groups. The results indicated there is great genetic difference among those specimen. Although No.30 is cultivar stirp, they still can be classified into the group of wild velvetleaf to show that the genetic difference between the No.30 and wild velvetleaf were relatively closer comparing with the other cultivar breeds.(3) 9 primers were selected from 91 ISSR primers, 82 DNA fragments were amplified from 48 samples. The average number of DNA bands was 9.11 per primer among them. The genetic similarity among all species ranged from 0.52~0.96, this indicated the genetic diversity was great. The cluster analysis based on ISSR markers showed that the 48 velvetleaf accessions could be classified into three groups at the level of D=0.71,the first group included 37 cultivated species, 2 wild species. The second group included 8 wild species, the third group only included 1 cultivated species. Division of velvetleaf germplasms was indicated that there were higher genetic diversity within velvetleaf cultivated and wild species .(4)There is a center of diversity of cultivar velvetleaf in North China, and a center of diversity of wild velvetleaf in the South .